CD2 Rekombinanter Antikörper

CD2 Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

241222B8

Kat-Nr. : 84108-5-RR

Synonyme

241222B8, CD2 molecule, Erythrocyte receptor, LFA 2, LFA 3 receptor

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  WB analysis using 84108-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84108-5-RR (CD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 84108-5-RR

  NK-92 cells were subjected to SDS PAGE followed by western blot with 84108-5-RR (CD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of Jurkat using 84108-5-RR

  Immunofluorescent analysis of (4% PFA) fixed Jurkat (positive) Raji (negative) cells using CD2 antibody (84108-5-RR, Clone: 241222B8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 84108-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84108-5-RR against Human CD2 were performed. The affinity constant is 0.494 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, NK-92 cells
• Erfolgreiche Detektion in IF/ICC: Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84108-5-RR bindet in WB, IF/ICC, ELISA CD2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD2 fusion protein Eg1271
• Vollständiger Name: CD2 molecule
• Berechnetes Molekulargewicht: 351 aa, 39 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC033583
• Gene symbol: CD2
• Gene ID (NCBI): 914
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD2 is a cell surface glycoprotein present on a majority of thymocytes, all mature T cells and subset of NK cells but not on B lymphocytes. It is a pan T-cell marker. CD2 interacts with lymphocyte function-associated antigen (LFA-3/CD58) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.