CD2 Rekombinanter Antikörper

CD2 Rekombinant Antikörper für WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

241548D7

Kat-Nr. : 84360-4-PBS

Synonyme

T-cell surface antigen CD2, Lymphocyte antigen 37, Ly-37, LFA-3 receptor, LFA-2

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  WB analysis using 84360-4-RR (same clone as 84360-4-PBS)

  CTLL-2 cells were subjected to SDS PAGE followed by western blot with 84360-4-RR (CD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84360-4-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01249-1

  Sandwich ELISA standard curve of MP01249-1, Mouse CD2 Recombinant Matched Antibody Pair - PBS only. 84360-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1403. 84360-4-PBS was HRP conjugated as the detection antibody. Range: 19.5-1250 pg/mL.

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  Sample test of MP01249-1

  The mean CD2 concentration was determined to be 3.0 ng/mL in CTLL-2 cell extract based on a 0.9 mg/mL extract load.

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  IHC staining of mouse thymus using 84360-4-RR (same clone as 84360-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 84360-4-RR (CD2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84360-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse thymus using 84360-4-RR (same clone as 84360-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 84360-4-RR (CD2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84360-4-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01249-1

  Cytometric bead array standard curve of MP01249-1, MOUSE CD2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84360-5-PBS. Detection antibody: 84360-4-PBS. Standard: Eg1403. Range: 0.313-40 ng/mL.

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Geprüfte Anwendungen

Produktinformation

84360-4-PBS bindet in WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test CD2 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: FusionProtein
• Vollständiger Name: CD2 antigen
• Berechnetes Molekulargewicht: 38 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: NM_013486.2
• Gene symbol: CD2
• Gene ID (NCBI): 12481
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD2 is a cell surface glycoprotein present on a majority of thymocytes, all mature T cells and subset of NK cells but not on B lymphocytes. It is a pan T-cell marker. CD2 interacts with lymphocyte function-associated antigen (LFA-3/CD58) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.