CD2 Rekombinanter Antikörper

CD2 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

241222E2

Kat-Nr. : 98134-1-RR

Synonyme

241222E2, CD2 molecule, Erythrocyte receptor, LFA 2, LFA 3 receptor

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  FC experiment of human PBMCs using 98134-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD2 Rabbit RecAb (98134-1-RR, Clone: 241222E2) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (CL647-65569, Clone: OKT3). Cells were not fixed. Lymphocytes were gated.

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  FC experiment of human PBMCs using 98134-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD2 Rabbit RecAb (98134-1-RR, Clone: 241222E2) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. Lymphocytes were gated.

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  Affinity and Kinetic Characterization of 98134-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98134-1-RR against Human CD2 were performed. The affinity constant is 0.418 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98134-1-RR bindet in FC CD2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD2 fusion protein Eg1271
• Vollständiger Name: CD2 molecule
• Berechnetes Molekulargewicht: 351 aa, 39 kDa
• GenBank-Zugangsnummer: BC033583
• Gene symbol: CD2
• Gene ID (NCBI): 914
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

CD2 is a cell surface glycoprotein present on a majority of thymocytes, all mature T cells and subset of NK cells but not on B lymphocytes. It is a pan T-cell marker. CD2 interacts with lymphocyte function-associated antigen (LFA-3/CD58) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.