CD20 Polyklonaler Antikörper

CD20 Polyklonal Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus, Ratte

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 31909-1-PBS

Synonyme

Membrane-spanning 4-domains subfamily A member 1, Lymphocyte antigen 44, B-lymphocyte antigen CD20, B-cell differentiation antigen Ly-44

---

• 

  WB analysis using 31909-1-AP (same clone as 31909-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 31909-1-AP (CD20 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31909-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 31909-1-AP (same clone as 31909-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 31909-1-AP (CD20 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31909-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 31909-1-AP (same clone as 31909-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 31909-1-AP (CD20 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31909-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat spleen using 31909-1-AP (same clone as 31909-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 31909-1-AP (CD20 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31909-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat spleen using 31909-1-AP (same clone as 31909-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 31909-1-AP (CD20 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31909-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse colon using 31909-1-AP (same clone as 31909-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse colon tissue using CD20 antibody (31909-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31909-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse colon using 31909-1-AP (same clone as 31909-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse colon tissue using CD20 antibody (31909-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31909-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse spleen using 31909-1-AP (same clone as 31909-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD20 antibody (31909-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31909-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse spleen using 31909-1-AP (same clone as 31909-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD20 antibody (31909-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31909-1-PBS in a different storage buffer formulation.

• 

  IF Staining of rat spleen using 31909-1-AP (same clone as 31909-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat spleen tissue using CD20 antibody (31909-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31909-1-PBS in a different storage buffer formulation.

• 

  IF Staining of rat spleen using 31909-1-AP (same clone as 31909-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat spleen tissue using CD20 antibody (31909-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31909-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

31909-1-PBS bindet in WB, IHC, IF-P, Indirect ELISA CD20 und zeigt Reaktivität mit Maus, Ratten

• Getestete Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CD20 fusion protein Ag36918
• Vollständiger Name: membrane-spanning 4-domains, subfamily A, member 1
• Berechnetes Molekulargewicht: 32 kDa
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: NM_007641
• Gene symbol: Ms4a1
• Gene ID (NCBI): 12482
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD20 is a 33-37 kDa transmembrane phosphoprotein. CD20 is a B-lymphocyte surface molecule that is widely expressed during B-cell ontogeny, from early pre-B-cell developmental stages until final differentiation into plasma cells. CD20 functions as a calcium-permeable cation channel. It is involved in the regulation of B-cell activation and proliferation. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B-cells.