CD20 Rekombinanter Antikörper

CD20 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Affe, human, nicht-humane Primaten

Anwendung

FC

Konjugation

APC-Cyanine7 Fluorescent Dye

CloneNo.

2H7

Kat-Nr. : AY7-FcA65575

Synonyme

2H7, B lymphocyte antigen CD20, B1, B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1

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  FC experiment of human PBMCs using AY7-FcA65575

  1x10^6 human PBMCs were surface stained with FcZero-rAb™ CoraLite® Plus 488 Anti-Human CD19 (4G7), and 5 ul APC-Cyanine7 Anti-Human CD20 (2H7) Rabbit IgG RecAb (AY7-FcA65575, Clone:2H7) or FcZero-rAb™ APC-Cyanine7 Rabbit IgG Isotype Control Recombinant Antibody (AY7-FcA98136, Clone: 240953C9). Cells were not fixed. Lymphocytes were gated.

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  FC experiment of human PBMCs using AY7-FcA65575

  1x10^6 human PBMCs were surface stained with 5 ul APC-Cyanine7 Anti-Human CD20 (2H7) Rabbit IgG RecAb (AY7-FcA65575, Clone: 2H7) (red) or FcZero-rAb™ APC-Cyanine7 Rabbit IgG Isotype Control Recombinant Antibody (AY7-FcA98136, Clone: 240953C9) (blue). Cells were not fixed. Lymphocytes were gated.

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  FC experiment of Cnyo PBMCs using AY7-FcA65575

  1x10^6 cynomolgus PBMCs were surface stained with APC Anti-Human CD3, and 5 ul APC-Cyanine7 Anti-Human CD20 (2H7) Rabbit IgG RecAb (AY7-FcA65575, Clone: 2H7) or FcZero-rAb™ APC-Cyanine7 Rabbit IgG Isotype Control Recombinant Antibody (AY7-FcA98136, Clone: 240953C9). Cells were not fixed.

• 

  FC experiment of Cnyo PBMCs using AY7-FcA65575

  1x10^6 cynomolgus PBMCs were surface stained with 5 ul APC-Cyanine7 Anti-Human CD20 (2H7) Rabbit IgG RecAb (AY7-FcA65575, Clone: 2H7) (red) or FcZero-rAb™ APC-Cyanine7 Rabbit IgG Isotype Control Recombinant Antibody (AY7-FcA98136, Clone: 240953C9) (blue). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs, cynomolgus PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

AY7-FcA65575 bindet in FC CD20 und zeigt Reaktivität mit Affe, human, nicht-humane Primaten

• Getestete Reaktivität: Affe, human, nicht-humane Primaten
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Humane tonsillare B-Zellen
• Vollständiger Name: membrane-spanning 4-domains, subfamily A, member 1
• Berechnetes Molekulargewicht: 297 aa, 33 kDa
• GenBank-Zugangsnummer: BC002807
• Gene symbol: CD20
• Gene ID (NCBI): 931
• Konjugation: APC-Cyanine7 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 650 nm / 778 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD20 is a 33-37 kDa transmembrane phosphoprotein belonging to the membrane-spanning 4A family (PMID: 3260267; 16785532). CD20 is a B-lymphocyte surface molecule that is widely expressed during B-cell ontogeny, from early pre-B-cell developmental stages until final differentiation into plasma cells (PMID: 7524522). CD20 functions as calcium-permeable cation channel (PMID: 7684739). It is involved in the regulation of B-cell activation, proliferation and differentiation (PMID: 7524522).