CD200 Monoklonaler Antikörper

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• KD/KO Validated

CD200 Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

5F3D6

Kat-Nr. : 66282-1-PBS

Synonyme

CD200 molecule, MOX1, MOX2, MRC, OX 2

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  WB analysis of fetal human brain using 66282-1-Ig (same clone as 66282-1-PBS)

  Untreated and PNGase F-treated lysates of fetal human brain tissue and human spleen tissue were subjected to SDS PAGE followed by western blot with 66282-1-Ig (CD200 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808). This data was developed using the same antibody clone with 66282-1-PBS in a different storage buffer formulation.

• 

  WB analysis of fetal human brain using 66282-1-Ig (same clone as 66282-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66282-1-Ig (CD200 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66282-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human spleen using 66282-1-Ig (same clone as 66282-1-PBS)

  human spleen tissue were subjected to SDS PAGE followed by western blot with 66282-1-Ig (CD200 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66282-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66282-1-Ig (same clone as 66282-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66282-1-Ig (CD200 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66282-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66282-1-Ig (same clone as 66282-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66282-1-Ig (CD200 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66282-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66282-1-PBS bindet in WB, IHC, Indirect ELISA CD200 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CD200 fusion protein Ag5045
• Vollständiger Name: CD200 molecule
• Berechnetes Molekulargewicht: 269 aa, 30 kDa
• Beobachtetes Molekulargewicht: 40-50 kDa
• GenBank-Zugangsnummer: BC022522
• Gene symbol: CD200
• Gene ID (NCBI): 4345
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD200, also known as OX2, is a type I transmembrane glycoprotein that belongs to the immunoglobulin superfamily (PMID: 3032785). It contains two extracellular immunoglobulin domains, a transmembrane and a cytoplasmic domain. CD200 is expressed on a variety of cell types, including thymocytes, B lymphocytes, a subset of T lymphocytes, follicular dendritic cells, neurons, and endothelial cells (PMID: 3032785; 10981966). CD200 binds to its receptor, CD200R, which is primarily expressed by myeloid and T cell lineage (PMID: 15187158). The CD200-CD200R interaction plays a role in immunosuppression and suppression of anti-tumor immune responses (PMID: 22020332).