CD200 Rekombinanter Antikörper

CD200 Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

240391A8

Kat-Nr. : 83437-5-RR

Synonyme

OX2, Mox2, CD200 antigen, 240391A8

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  WB analysis using 83437-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83437-5-RR (CD200 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 83437-5-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83437-5-RR (CD200 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 83437-5-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83437-5-RR (CD200 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 83437-5-RR

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 83437-5-RR (CD200 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 83437-5-RR

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 83437-5-RR (CD200 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  Affinity and Kinetic Characterization of 83437-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83437-5-RR against Mouse CD200 were performed. The affinity constant is 28.8 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Cerebellum-Gewebe, Maushirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe, Maus-Cerebellum-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83437-5-RR bindet in WB, IHC, ELISA CD200 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Fusionsprotein
• Vollständiger Name: CD200 antigen
• Berechnetes Molekulargewicht: 31 kDa
• Beobachtetes Molekulargewicht: 40 kDa
• GenBank-Zugangsnummer: /
• Gene symbol: Cd200
• Gene ID (NCBI): 17470
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD200, also known as OX2, is a type I transmembrane glycoprotein that belongs to the immunoglobulin superfamily (PMID: 3032785). It contains two extracellular immunoglobulin domains, a transmembrane and a cytoplasmic domain. CD200 is expressed on a variety of cell types, including thymocytes, B lymphocytes, a subset of T lymphocytes, follicular dendritic cells, neurons, and endothelial cells (PMID: 3032785; 10981966). CD200 binds to its receptor, CD200R, which is primarily expressed by myeloid and T cell lineage (PMID: 15187158). The CD200-CD200R interaction plays a role in immunosuppression and suppression of anti-tumor immune responses (PMID: 22020332).