CD200 Rekombinanter Antikörper

CD200 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

241347E1

Kat-Nr. : 98091-1-RR

Synonyme

OX 2, MRC, MOX2, MOX1, CD200 molecule

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  FC experiment of human PBMCs using 98091-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD200 Rabbit Recombinant Antibody (98091-1-RR, Clone:241347E1) or 0.25 ug Isotype Control and PE-conjugated Goat Anti-Rabbit IgG. Cell were then stained with CoraLite® Plus 647 Anti-Human CD19 (4G7). Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 98091-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD200 Rabbit Recombinant Antibody (98091-1-RR, Clone:241347E1) (red) or 0.25 ug Isotype Control (blue) and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 98091-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD200 Rabbit Recombinant Antibody (98091-1-RR, Clone:241347E1) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), and 0.25 ug Isotype Control, and 0.25 ug CoraLite® Plus 647 Anti-Human CD19 (4G7) Mouse IgG2a Recombinant Antibody (CL647-65562, Clone: 4G7). Cells were not fixed.

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  Affinity and Kinetic Characterization of 98091-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98091-1-RR against Human CD200 were performed. The affinity constant is 1.15 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98091-1-RR bindet in FC CD200 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: CD200 molecule
• Berechnetes Molekulargewicht: 269 aa, 30 kDa
• GenBank-Zugangsnummer: BC022522
• Gene symbol: CD200
• Gene ID (NCBI): 4345
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

CD200, also known as OX2, is a type I transmembrane glycoprotein that belongs to the immunoglobulin superfamily (PMID: 3032785). It contains two extracellular immunoglobulin domains, a transmembrane and a cytoplasmic domain. CD200 is expressed on a variety of cell types, including thymocytes, B lymphocytes, a subset of T lymphocytes, follicular dendritic cells, neurons, and endothelial cells (PMID: 3032785; 10981966). CD200 binds to its receptor, CD200R, which is primarily expressed by myeloid and T cell lineage (PMID: 15187158). The CD200-CD200R interaction plays a role in immunosuppression and suppression of anti-tumor immune responses (PMID: 22020332).