CD204 Rekombinanter Antikörper

CD204 Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

251367C1

Kat-Nr. : 86552-3-RR

Synonyme

Macrophage acetylated LDL receptor I and II, macrophage scavenger receptor 1, Macrophage scavenger receptor types I and II, Msr1, Scavenger receptor type A

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  WB analysis of J774A.1 using 86552-3-RR

  J774A.1 cells were subjected to SDS PAGE followed by western blot with 86552-3-RR (Msr1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse lung using 86552-3-RR

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 86552-3-RR (CD204 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse lung using 86552-3-RR

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 86552-3-RR (CD204 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse small intestine using 86552-3-RR

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 86552-3-RR (CD204 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: J774A.1 cells
• Erfolgreiche Detektion in IHC: Mauslungengewebe, Maus-Dünndarmgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86552-3-RR bindet in WB, IHC, ELISA CD204 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD204 fusion protein Eg4727
• Vollständiger Name: macrophage scavenger receptor 1
• Berechnetes Molekulargewicht: 50 kDa
• Beobachtetes Molekulargewicht: 70-90 kDa
• GenBank-Zugangsnummer: NM_001113326.1
• Gene symbol: Msr1
• Gene ID (NCBI): 20288
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MSR1, also known as scavenger receptor-A (SR-A) or cluster of differentiation 204 (CD204), was first described in 1979 by Brown and Goldstein. MSR1 is highly expressed in lung, arteries, and adipose tissue.MSR1 is predominantly expressed in various macrophage cell types and is mainly altered in macrophage-associated physiological and pathological processes including atherosclerosis, Alzheimer's disease, host defence, and cancer.