CD206 Rekombinanter Antikörper

CD206 Rekombinant Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human, Ratte

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1G15

Kat-Nr. : 81525-1-PBS

Synonyme

1G15, CLEC13D, CLEC13DL, C-type lectin domain family 13 member D, C-type lectin domain family 13 member D-like

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  WB analysis using 81525-1-RR (same clone as 81525-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81525-1-RR (CD206 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81525-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 81525-1-RR (same clone as 81525-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 81525-1-RR (CD206 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81525-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 81525-1-RR (same clone as 81525-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 81525-1-RR (CD206 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81525-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 81525-1-RR (same clone as 81525-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 81525-1-RR (CD206 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81525-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 81525-1-RR (same clone as 81525-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 81525-1-RR (CD206 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81525-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 81525-1-RR (same clone as 81525-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 81525-1-RR (CD206 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81525-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 81525-1-RR (same clone as 81525-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 81525-1-RR (CD206 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81525-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 81525-1-RR (same clone as 81525-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 81525-1-RR (CD206 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81525-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 81525-1-RR (same clone as 81525-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 81525-1-RR (CD206 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81525-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 81525-1-RR (same clone as 81525-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 81525-1-RR (CD206 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81525-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 81525-1-RR (same clone as 81525-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 81525-1-RR (CD206 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81525-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 81525-1-RR (same clone as 81525-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 81525-1-RR (CD206 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81525-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 81525-1-RR (same clone as 81525-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 81525-1-RR (CD206 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81525-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung using 81525-1-RR (same clone as 81525-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 81525-1-RR (CD206 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81525-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung using 81525-1-RR (same clone as 81525-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 81525-1-RR (CD206 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81525-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human placenta using 81525-1-RR (same clone as 81525-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using CD206 antibody (81525-1-RR, Clone: 1G15 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81525-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human placenta using 81525-1-RR (same clone as 81525-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using CD206 antibody (81525-1-RR, Clone: 1G15 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81525-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

81525-1-PBS bindet in WB, IHC, IF-P, Indirect ELISA CD206 und zeigt Reaktivität mit Hausschwein, human, Ratten

• Getestete Reaktivität: Hausschwein, human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: mannose receptor, C type 1
• Berechnetes Molekulargewicht: 166 kDa
• Beobachtetes Molekulargewicht: 170 kDa
• GenBank-Zugangsnummer: NM_002438
• Gene symbol: CD206
• Gene ID (NCBI): 4360
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD206, also named as MMR, CLEC13D and MRC1, is a type I membrane receptor that mediates the endocytosis of glycoproteins by macrophages. CD206 has been shown to bind high-mannose structures on the surface of potentially pathogenic viruses, bacteria, and fungi so that they can be neutralized by phagocytic engulfment. CD206 is a 170 kDa transmembrane protein which contains 5 domains: an amino-terminal cysteine-rich region, a fibronectin type II repeat, a series of eight tandem lectin-like carbohydrate recognition domains (responsible for the recognition of mannose and fucose), a transmembrane domain, and an intracellular carboxy-terminal tail. It is expressed on most tissue macrophages, in vitro derived dendritic cells, lymphatic and sinusoidal endothelia.