CD206 Rekombinanter Antikörper

CD206 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

IF, FC

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Publikationen(1)

CloneNo.

240344D12

Kat-Nr. : CL488-98031

Synonyme

Mrc1, MR, MMR, mannose receptor, C type 1, 240344D12

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  FC experiment of mouse peritoneal macrophages using CL488-98031

  1x10^6 mouse peritoneal macrophages were surface stained with APC Anti-Mouse CD107b / LAMP2, and 0.25 ug CoraLite® Plus 488 Anti-Mouse CD206 Rabbit RecAb (CL488-98031, Clone:240344D12) or CoraLite® Plus 488 Rabbit IgG Isotype Control RecAb (CL488-98136, Clone: 240953C9). Cells were incubated with FC Receptor Block prior to staining and fixing. Cells were not fixed.

• 

  FC experiment of mouse peritoneal macrophages using CL488-98031

  1x10^6 mouse peritoneal macrophages were surface stained with 0.25 ug CoraLite® Plus 488 Anti-Mouse CD206 Rabbit RecAb (CL488-98031, Clone: 240344D12) (red), or CoraLite® Plus 488 Rabbit IgG Isotype Control RecAb (CL488-98136, Clone: 240953C9) (blue). Cells were incubated with FC Receptor Block prior to staining and fixing. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: peritoneale Makrophagen der Maus

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below
• FC: See 1 publications below

Produktinformation

CL488-98031 bindet in IF, FC CD206 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: mannose receptor, C type 1
• Berechnetes Molekulargewicht: 165 kDa
• GenBank-Zugangsnummer: NM_008625.2
• Gene symbol: CD206
• Gene ID (NCBI): 17533
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD206, also named as MMR, CLEC13D and MRC1, is a type I membrane receptor that mediates the endocytosis of glycoproteins by macrophages. CD206 has been shown to bind high-mannose structures on the surface of potentially pathogenic viruses, bacteria, and fungi so that they can be neutralized by phagocytic engulfment. CD206 is a 170 kDa transmembrane protein which contains 5 domains: an amino-terminal cysteine-rich region, a fibronectin type II repeat, a series of eight tandem lectin-like carbohydrate recognition domains (responsible for the recognition of mannose and fucose), a transmembrane domain, and an intracellular carboxy-terminal tail. It is expressed on most tissue macrophages, in vitro derived dendritic cells, lymphatic and sinusoidal endothelia.

Publikationen

Species	Application	Title
mouse	IF,FC	Biomacromolecules; Ganoderma lucidum Polysaccharide/carboxymethyl Chitosan Hydrogels Modulate Macrophage Polarization for Wound Healing; Authors - Yu Zou