CD206 Monoklonaler Antikörper

CD206 Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / IgG1, kappa

Getestete Reaktivität

human

Anwendung

FC

Konjugation

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

15-2

Kat-Nr. : CL647-65155

Synonyme

15-2, CLEC13D, CLEC13DL, C-type lectin domain family 13 member D, C-type lectin domain family 13 member D-like

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  FC experiment of human PBMCs using CL647-65155

  1x10^6 GM-CSF treated human PBMCs were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD206 (15-2) (CL647-65155, Clone:15-2) (red) or CoraLite® Plus 647 Mouse IgG1 Isotype Control (MOPC-21) (CL647-65124, Clone: MOPC-21) (blue). 1x10^6 untreated human PBMCs were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD206 (15-2) (CL647-65155, Clone: 15-2) (black, dashed). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: GM-CSF treated human PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

CL647-65155 bindet in FC CD206 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Gereinigter humaner Mannose-Rezeptor
• Vollständiger Name: mannose receptor, C type 1
• Berechnetes Molekulargewicht: 166 kDa
• GenBank-Zugangsnummer: NM_002438
• Gene symbol: CD206
• Gene ID (NCBI): 4360
• Konjugation: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Form: Liquid
• Reinigungsmethode: Mit Protein-A-Affinitätschromatographie gereinigt
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD206, also named as MMR, CLEC13D and MRC1, is a type I membrane receptor that mediates the endocytosis of glycoproteins by macrophages. CD206 has been shown to bind high-mannose structures on the surface of potentially pathogenic viruses, bacteria, and fungi so that they can be neutralized by phagocytic engulfment. CD206 is a 170 kDa transmembrane protein which contains 5 domains: an amino-terminal cysteine-rich region, a fibronectin type II repeat, a series of eight tandem lectin-like carbohydrate recognition domains (responsible for the recognition of mannose and fucose), a transmembrane domain, and an intracellular carboxy-terminal tail. It is expressed on most tissue macrophages, in vitro derived dendritic cells, lymphatic and sinusoidal endothelia.