CD206 Monoklonaler Antikörper

CD206 Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / IgG1, kappa

Getestete Reaktivität

human

Anwendung

FC

Konjugation

FITC Plus Fluorescent Dye

Publikationen(13)

CloneNo.

15-2

Kat-Nr. : FITC-65155

Synonyme

hMR, C-type lectin domain family 13 member D-like, C-type lectin domain family 13 member D, CLEC13DL, CLEC13D

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  FC experiment of human PBMCs using FITC-65155

  1x10^6 GM-CSF treated human PBMCs were surface stained with 5 ul FITC Anti-Human CD206 (15-2) (FITC-65155, Clone:15-2) (red) or FITC Plus Mouse IgG1 Isotype Control (MOPC-21) (FITC-65124, Clone: MOPC-21) (blue). 1x10^6 untreated human PBMCs were surface stained with 5 ul FITC Anti-Human CD206 (15-2) (FITC-65155, Clone:15-2) (black, dashed). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: GM-CSF treated human PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• FC: See 12 publications below

Produktinformation

FITC-65155 bindet in FC CD206 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Gereinigter humaner Mannose-Rezeptor
• Vollständiger Name: mannose receptor, C type 1
• Berechnetes Molekulargewicht: 166 kDa
• GenBank-Zugangsnummer: NM_002438
• Gene symbol: CD206
• Gene ID (NCBI): 4360
• Konjugation: FITC Plus Fluorescent Dye
• Excitation/Emission maxima wavelengths: 495 nm / 524 nm
• Form: Liquid
• Reinigungsmethode: Der gereinigte Antikörper ist mit Fluoresceinisothiocyanat (FITC) unter optimalen Bedingungen konjugiert. Das Konjugat wird mit Größenausschluss-Chromatographie gereinigt.
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD206, also named as MMR, CLEC13D and MRC1, is a type I membrane receptor that mediates the endocytosis of glycoproteins by macrophages. CD206 has been shown to bind high-mannose structures on the surface of potentially pathogenic viruses, bacteria, and fungi so that they can be neutralized by phagocytic engulfment. CD206 is a 170 kDa transmembrane protein which contains 5 domains: an amino-terminal cysteine-rich region, a fibronectin type II repeat, a series of eight tandem lectin-like carbohydrate recognition domains (responsible for the recognition of mannose and fucose), a transmembrane domain, and an intracellular carboxy-terminal tail. It is expressed on most tissue macrophages, in vitro derived dendritic cells, lymphatic and sinusoidal endothelia.

Publikationen

Species	Application	Title
human	FC	Biochim Biophys Acta Mol Basis Dis; Extracellular ATP (eATP) inhibits the progression of endometriosis and enhances the immune function of macrophages; Authors - Ling Zhou
	FC	Front Immunol; Chamaejasmenin B, an Inhibitor for Metastatic Outgrowth, Reversed M2-Dominant Macrophage Polarization in Breast Tumor Microenvironment.; Authors - Qi Li
	FC	Pharm Biol; Schizandrin A ameliorates cognitive functions via modulating microglial polarisation in Alzheimer's disease mice.; Authors - Qi Wang
	FC	Cancer Sci; Homeobox A7 promotes esophageal squamous cell carcinoma progression through C-C motif chemokine ligand 2-mediated tumor-associated macrophage recruitment; Authors - Anqi Feng
human	FC	J Biochem Mol Toxicol; KLF4-induced upregulation of SOCS1 ameliorates myocardial ischemia/reperfusion injury by attenuating AC16 cardiomyocyte damage and enhancing M2 macrophage polarization; Authors - Jiming Zhou
	FC	Authors -