CD206 Monoklonaler Antikörper

CD206 Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / IgG1, kappa

Getestete Reaktivität

human

Anwendung

FC

Konjugation

FITC Fluorescent Dye

Publikationen

2

CloneNo.

15-2

Kat-Nr. : FITC-65155

Synonyme

CD206, CLEC13D, Macrophage mannose receptor 1, mannose receptor, C type 1, MMR, MRC1

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Galerie der Validierungsdaten

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  FC experiment of human monocyte-derived dendritic cells using FITC-65155

  Human monocyte-derived dendritic cells (monocytes stimulated with GM-CSF and IL-4) were surface stained with 4 ul FITC Anti-Human CD206 (FITC-65155, Clone: 15-2) (green) or FITC-Mouse IgG1 isotype control (black). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane, aus Monozyten gewonnene dendritische Zellen Zellen

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 4 µl per 10^6 cells in a 100 µl suspension or 4 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• FC: See 2 publications below

Produktinformation

FITC-65155 bindet in FC CD206 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Gereinigter humaner Mannose-Rezeptor
• Vollständiger Name: mannose receptor, C type 1
• Berechnetes Molekulargewicht: 166 kDa
• GenBank-Zugangsnummer: NM_002438
• Gene symbol: MRC1
• Gene ID (NCBI): 4360
• Konjugation: FITC Fluorescent Dye
• Excitation/Emission maxima wavelengths: 494 nm / 520 nm
• Form: Liquid
• Reinigungsmethode: Der gereinigte Antikörper ist mit Fluoresceinisothiocyanat (FITC) unter optimalen Bedingungen konjugiert. Das Konjugat wird mit Größenausschluss-Chromatographie gereinigt.
• Lagerungspuffer: PBS mit 0,1% Natriumazid.
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD206, also named as MMR, CLEC13D and MRC1, is a type I membrane receptor that mediates the endocytosis of glycoproteins by macrophages. CD206 has been shown to bind high-mannose structures on the surface of potentially pathogenic viruses, bacteria, and fungi so that they can be neutralized by phagocytic engulfment. CD206 is a 170 kDa transmembrane protein which contains 5 domains: an amino-terminal cysteine-rich region, a fibronectin type II repeat, a series of eight tandem lectin-like carbohydrate recognition domains (responsible for the recognition of mannose and fucose), a transmembrane domain, and an intracellular carboxy-terminal tail. It is expressed on most tissue macrophages, in vitro derived dendritic cells, lymphatic and sinusoidal endothelia.

Publikationen

Species	Application	Title
mouse	FC	Front Immunol; Chamaejasmenin B, an Inhibitor for Metastatic Outgrowth, Reversed M2-Dominant Macrophage Polarization in Breast Tumor Microenvironment.; Authors - Qi Li
	FC	Pharm Biol; Schizandrin A ameliorates cognitive functions via modulating microglial polarisation in Alzheimer's disease mice.; Authors - Qi Wang