CD207 Polyklonaler Antikörper

CD207 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 11841-1-AP

Synonyme

Langerin, CD207 molecule, langerin, CLEC4K, C-type lectin domain family 4 member K

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  WB analysis of human skin using 11841-1-AP

  human skin tissue were subjected to SDS PAGE followed by western blot with 11841-1-AP (Langerin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skin using 11841-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 11841-1-AP (Langerin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin cancer using 11841-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 11841-1-AP (Langerin antibody) at dilution of 1:6000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 11841-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 11841-1-AP (Langerin antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hautgewebe, Maushautgewebe
• Erfolgreiche Detektion in IHC: humanes Hautkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:3000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

11841-1-AP bindet in WB, IHC, ELISA CD207 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CD207 fusion protein Ag2479
• Vollständiger Name: CD207 molecule, langerin
• Berechnetes Molekulargewicht: 328 aa, 37 kDa
• Beobachtetes Molekulargewicht: 40 kDa
• GenBank-Zugangsnummer: BC022278
• Gene symbol: CD207
• Gene ID (NCBI): 50489
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD207 protein (also known as langerin protein) is encoded by the CD207 gene and is a type of C-type lectin receptor that is specifically expressed on the surface of LCs. It is one of the specific immunohistochemical markers of LCs (PMID: 37383183, PMID: 28109175). CD207 plays an important role in a series of non-classical antigen processing processes, such as forming Birbeck granules and processing and transporting them to local lymphoid tissue, and inducing LC maturation (PMID: 32326440).

Publikationen

Species	Application	Title
		Elife; Cryo-electron tomography of Birbeck granules reveals the molecular mechanism of langerin lattice formation.; Authors - Toshiyuki Oda