CD21 Rekombinanter Antikörper

CD21 Rekombinant Antikörper für FC

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

BU32

Kat-Nr. : 65512-1-MR

Synonyme

BU32, C3DR, Complement C3d receptor, Complement receptor type 2, CR2

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  FC experiment of human PBMCs using 65512-1-MR

  1x10^6 human PBMCs were surface stained with 0.1 ug Anti-Human CD21 Mouse Recombinant Antibody (65512-1-MR, Clone:BU32) or Mouse IgG1 Isotype Control and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were then stained with CL488-Conjugated Anti-Human CD3. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 65512-1-MR

  1x10^6 human PBMCs were surface stained with 0.1 ug Anti-Human CD21 Mouse Recombinant Antibody (65512-1-MR, Clone:BU32) or Mouse IgG1 Isotype Control and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 65512-1-MR

  1x10^6 human PBMCs were surface stained with 0.1 ug Anti-Human CD21 Mouse Recombinant Antibody (65512-1-MR, Clone:BU32) (left) or 0.1 ug Anti-Human CD21 Mouse Monoclonal Antibody (Clone:BU32) (center and right) and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were then stained with CL488-Conjugated Anti-Human CD3. Cells were not fixed. Lymphocytes were gated.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

65512-1-MR bindet in FC CD21 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: k.A.
• Vollständiger Name: complement component (3d/Epstein Barr virus) receptor 2
• Berechnetes Molekulargewicht: 1092 aa, 119 kDa
• GenBank-Zugangsnummer: BC136394
• Gene symbol: CD21
• Gene ID (NCBI): 1380
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Stable for one year after shipment.

Hintergrundinformationen

CD21, also known as complement receptor type 2 (CR2), complement C3d receptor, and Epstein-Barr virus receptor, is a transmembrane protein that contains a small cytoplasmic domain, a transmembrane region and an extracellular domain consisting of 15 tandem short consensus repeat sequences (PMID: 6230668; 2551147). It is expressed on B cells, follicular dendritic cells, thymocytes and a subset of peripheral T cells (PMID: 26119182). CD21 binds complement fragments C3d, C3dg and iC3b and acts as a receptor for the Epstein-Barr virus (PMID: 7753047). On B cells, together with CD19 and CD81, CD21 forms a complex that functions as a co-receptor to the BCR (PMID: 7542009). It is involved in B cells activation and functions.