CD21/CD35 Rekombinanter Antikörper

CD21/CD35 Rekombinant Antikörper für WB, IHC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus, Ratte

Anwendung

WB, IHC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241957E6

Kat-Nr. : 84565-2-PBS

Synonyme

CD21, Cr2, 241957E6

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  WB analysis using 84565-2-RR (same clone as 84565-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84565-2-RR (CD21/CD35 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84565-2-PBS in a different storage buffer formulation.

• 

  WB analysis using 84565-2-RR (same clone as 84565-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84565-2-RR (CD21 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84565-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 84565-2-RR (same clone as 84565-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84565-2-RR (CD21 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84565-2-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 84565-2-RR (same clone as 84565-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84565-2-RR (CD21 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84565-2-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP01407-1

  Cytometric bead array standard curve of MP01407-1, MOUSE CD21 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84565-2-PBS. Detection antibody: 84565-3-PBS. Standard: Eg1396. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 84565-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84565-2-RR against Mouse CD21/CD35 were performed. The affinity constant is 4.84 nM.

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Geprüfte Anwendungen

Produktinformation

84565-2-PBS bindet in WB, IHC, Cytometric bead array, Indirect ELISA CD21/CD35 und zeigt Reaktivität mit Maus, Ratten

• Getestete Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: complement receptor 2
• Berechnetes Molekulargewicht: 113kDa
• Beobachtetes Molekulargewicht: 160-210 kDa
• GenBank-Zugangsnummer: AAA37448.1
• Gene symbol: Cr2
• Gene ID (NCBI): 12902
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD21, also known as complement receptor type 2 (CR2), complement C3d receptor, and Epstein-Barr virus receptor, is a transmembrane protein that contains a small cytoplasmic domain, a transmembrane region and an extracellular domain consisting of 15 short consensus repeats (SCRs) (PMID: 6230668; 2551147). CD21 binds complement fragments C3d, C3dg and iC3b and acts as a receptor for the Epstein-Barr virus (PMID: 7753047). CD35, also known as complement receptor type 1 (CR1) or C3b/C4b receptor, binds C3b and C4b. The murine Cr2 gene encodes for both CD21 and CD35 (PMID: 2139460; 19559484). Mouse CD35 comprises 21 SCRs while mouse CD21 is identical to mouse CD35 except that it lacks the first 6 SCRs. CD21/CD35 forms trimolecular complexes with CD19 and CD81 and plays roles in the regulation of humoral immune responses (PMID: 11043778).