CD21 Monoklonaler Antikörper

CD21 Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / IgG1, kappa

Getestete Reaktivität

human

Anwendung

FC

Konjugation

CoraLite® Plus 405 Fluorescent Dye

CloneNo.

BU32

Kat-Nr. : CL405-65198

Synonyme

BU32, C3DR, Complement C3d receptor, Complement receptor type 2, CR2

---

• 

  FC experiment of human PBMCs using CL405-65198

  1X10^6 human PBMCs were surface co-stained with FITC Anti-Human CD19 and 5 ul CoraLite® Plus 405 Anti-Human CD21 (CL405-65198, Clone:BU32) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using CL405-65198

  1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 405 Anti-Human CD21 (CL405-65198, Clone:BU32) (red) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

CL405-65198 bindet in FC CD21 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: k.A.
• Vollständiger Name: complement component (3d/Epstein Barr virus) receptor 2
• Berechnetes Molekulargewicht: 1092 aa, 119 kDa
• GenBank-Zugangsnummer: BC136394
• Gene symbol: CD21
• Gene ID (NCBI): 1380
• Konjugation: CoraLite® Plus 405 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 399 nm / 422 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD21, also known as complement receptor type 2 (CR2), complement C3d receptor, and Epstein-Barr virus receptor, is a transmembrane protein that contains a small cytoplasmic domain, a transmembrane region and an extracellular domain consisting of 15 tandem short consensus repeat sequences (PMID: 6230668; 2551147). It is expressed on B cells, follicular dendritic cells, thymocytes and a subset of peripheral T cells (PMID: 26119182). CD21 binds complement fragments C3d, C3dg and iC3b and acts as a receptor for the Epstein-Barr virus (PMID: 7753047). On B cells, together with CD19 and CD81, CD21 forms a complex that functions as a co-receptor to the BCR (PMID: 7542009). It is involved in B cells activation and functions.