CD22 Monoklonaler Antikörper

CD22 Monoklonal Antikörper für WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

7A2F1

Kat-Nr. : 66103-1-PBS

Synonyme

7A2F1, B cell receptor CD22, B-cell receptor CD22, BL-CAM, B-lymphocyte cell adhesion molecule

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  WB analysis of Ramos using 66103-1-Ig (same clone as 66103-1-PBS)

  Ramos, Daudi, and Raji cells were subjected to SDS PAGE followed by western blot with 66103-1-Ig (CD22 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66103-1-PBS in a different storage buffer formulation.

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  WB analysis of Raji using 66103-1-Ig (same clone as 66103-1-PBS)

  Untreated and PNGase F-treated lysates of Raji cells were subjected to SDS PAGE followed by western blot with 66103-1-Ig (CD22 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808). This data was developed using the same antibody clone with 66103-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66103-1-Ig (same clone as 66103-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66103-1-Ig (CD22 antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66103-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66103-1-Ig (same clone as 66103-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66103-1-Ig (CD22 antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66103-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66103-1-Ig (same clone as 66103-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66103-1-Ig (CD22 antibody) at dilution of 1:20000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66103-1-PBS in a different storage buffer formulation.

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  IHC staining of human appendicitis using 66103-1-Ig (same clone as 66103-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66103-1-Ig (CD22 antibody) at dilution of 1:6400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66103-1-PBS in a different storage buffer formulation.

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  IHC staining of human appendicitis using 66103-1-Ig (same clone as 66103-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66103-1-Ig (CD22 antibody) at dilution of 1:6400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66103-1-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 66103-1-Ig (same clone as 66103-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD22 antibody (66103-1-Ig, Clone: 7A2F1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66103-1-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 66103-1-Ig (same clone as 66103-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD22 antibody (66103-1-Ig, Clone: 7A2F1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66103-1-PBS in a different storage buffer formulation.

• 

  IF Staining of Raji using 66103-1-Ig (same clone as 66103-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Raji cells using CD22 antibody (66103-1-Ig, Clone: 7A2F1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66103-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50311-4

  Cytometric bead array standard curve of MP50311-4, CD22 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66103-1-PBS. Detection antibody: 66103-6-PBS. Standard:Ag17986. Range: 0.781-100 ng/mL.

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Geprüfte Anwendungen

Produktinformation

66103-1-PBS bindet in WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA CD22 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CD22 fusion protein Ag17986
• Vollständiger Name: CD22 molecule
• Berechnetes Molekulargewicht: 847 aa, 95 kDa
• Beobachtetes Molekulargewicht: 135 kDa
• GenBank-Zugangsnummer: BC109306
• Gene symbol: CD22
• Gene ID (NCBI): 933
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD22, also known as Siglec-2 (sialic acid binding Ig-like lectin 2) or BL-CAM (B-lymphocyte cell adhesion molecule), is a 130-140 kDa, B-cell restricted, type I transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. The expression of CD22 is developmentally regulated. It is expressed at low levels in the cytoplasm of pro-B and pre-B cells and present on the cell surface only at mature stages of B-cell differentiation. Cell surface expression is lost during terminal differentiation into plasma cell and after B-cell activation. CD22 is an inhibitory receptor for B-cell receptor (BCR) signalling, preferentially binds to alpha-2,6-linked sialic acid and mediates B-cell B-cell interactions. It plays a crucial role in activation and differentiation of the B-cell.