CD22 Rekombinanter Antikörper

CD22 Rekombinant Antikörper für IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

250078E8

Kat-Nr. : 86128-2-RR

Synonyme

B-cell receptor CD22, BL-CAM, B-lymphocyte cell adhesion molecule, Lyb-8, Sialic acid-binding Ig-like lectin 2

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  IF Staining of A20 using 86128-2-RR

  Immunofluorescent analysis of (4% PFA) fixed A20 cells using CD22 antibody (86128-2-RR, Clone: 250078E8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 86128-2-RR

  Biolayer interferometry (BLl) kinetic assays of 86128-2-RR against Human CD22 were performed. The affinity constant is 0.63 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: A20 cells

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86128-2-RR bindet in IF/ICC, ELISA CD22 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD22 fusion protein Eg1324
• Vollständiger Name: CD22 antigen
• Berechnetes Molekulargewicht: 97kDa
• GenBank-Zugangsnummer: NM_001043317.2
• Gene symbol: Cd22
• Gene ID (NCBI): 12483
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD22, also known as Siglec-2 (sialic acid binding Ig-like lectin 2) or BL-CAM (B-lymphocyte cell adhesion molecule), is a 130-140 kDa, B-cell restricted, type I transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. The expression of CD22 is developmentally regulated. It is expressed at low levels in the cytoplasm of pro-B and pre-B cells and present on the cell surface only at mature stages of B-cell differentiation. Cell surface expression is lost during terminal differentiation into plasma cell and after B-cell activation. CD22 is an inhibitory receptor for B-cell receptor (BCR) signalling, preferentially binds to alpha-2,6-linked sialic acid and mediates B-cell B-cell interactions. It plays a crucial role in activation and differentiation of the B-cell.