CD22 Rekombinanter Antikörper

CD22 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

250078D10

Kat-Nr. : 98485-2-RR

Synonyme

B-cell receptor CD22, BL-CAM, B-lymphocyte cell adhesion molecule, Lyb-8, Sialic acid-binding Ig-like lectin 2

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  FC experiment of mouse splenocytes using 98485-2-RR

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD22 Rabbit RecAb (98485-2-RR, Clone: 250078D10) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 488 Anti-Mouse CD19 (1D3) (CL488-65290, Clone: 1D3). Cells were not fixed.

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  FC experiment of mouse splenocytes using 98485-2-RR

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD22 Rabbit RecAb (98485-2-RR, Clone:250078D10) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 488 Anti-Mouse CD19 (1D3) (CL488-65290, Clone: 1D3). Cells were not fixed.

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  Affinity and Kinetic Characterization of 98485-2-RR

  Biolayer interferometry (BLl) kinetic assays of 98485-2-RR against Mouse CD22 were performed. The affinity constant is 17.4 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Maus-Splenozyten

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98485-2-RR bindet in FC CD22 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD22 fusion protein Eg1324
• Vollständiger Name: CD22 antigen
• Berechnetes Molekulargewicht: 97kDa
• GenBank-Zugangsnummer: NM_001043317.2
• Gene symbol: Cd22
• Gene ID (NCBI): 12483
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

CD22, also known as Siglec-2 (sialic acid binding Ig-like lectin 2) or BL-CAM (B-lymphocyte cell adhesion molecule), is a 130-140 kDa, B-cell restricted, type I transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. The expression of CD22 is developmentally regulated. It is expressed at low levels in the cytoplasm of pro-B and pre-B cells and present on the cell surface only at mature stages of B-cell differentiation. Cell surface expression is lost during terminal differentiation into plasma cell and after B-cell activation. CD22 is an inhibitory receptor for B-cell receptor (BCR) signalling, preferentially binds to alpha-2,6-linked sialic acid and mediates B-cell B-cell interactions. It plays a crucial role in activation and differentiation of the B-cell.