CD22 Monoklonaler Antikörper

CD22 Monoklonal Antikörper für IF-P

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

IF-P

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

7A2F1

Kat-Nr. : CL594-66103

Synonyme

B cell receptor CD22, BL CAM, CD22, CD22 molecule, FLJ22814, SIGLEC 2, SIGLEC2, T cell surface antigen Leu 14



Geprüfte Anwendungen

Erfolgreiche Detektion in IF-Phumanes Tonsillitisgewebe

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL594-66103 bindet in IF-P CD22 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Maus / IgG2a
Klonalität Monoklonal
Typ Antikörper
Immunogen CD22 fusion protein Ag17986
Vollständiger Name CD22 molecule
Berechnetes Molekulargewicht 847 aa, 95 kDa
GenBank-ZugangsnummerBC109306
Gene symbol CD22
Gene ID (NCBI) 933
Konjugation CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD22, also known as Siglec-2 (sialic acid binding Ig-like lectin 2) or BL-CAM (B-lymphocyte cell adhesion molecule), is a 130-140 kDa, B-cell restricted, type I transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. The expression of CD22 is developmentally regulated. It is expressed at low levels in the cytoplasm of pro-B and pre-B cells and present on the cell surface only at mature stages of B-cell differentiation. Cell surface expression is lost during terminal differentiation into plasma cell and after B-cell activation. CD22 is an inhibitory receptor for B-cell receptor (BCR) signalling, preferentially binds to alpha-2,6-linked sialic acid and mediates B-cell B-cell interactions. It plays a crucial role in activation and differentiation of the B-cell. And this antibody is conjugated with CL594, Ex/Em 593 nm/614 nm.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
IF protocol for CL594 CD22 antibody CL594-66103Protokoll herunterladen
STANDARD-PROTOKOLLE
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