CD223 Monoklonaler Antikörper

CD223 Monoklonal Antikörper für FC

Wirt / Isotyp

Ratte / IgG1, kappa

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

C9B7W

Kat-Nr. : 65098-1-Ig

Synonyme

CD223, LAG 3, Lag3, Ly66, lymphocyte activation gene 3

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Galerie der Validierungsdaten

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  FC experiment of BALB/c mouse splenocytes using 65098-1-Ig

  1X10^6 Con-A stimulated BALB/c mouse splenocytes were surface co-stained with CoraLite® Plus 647-Conjugated Anti-Mouse CD3 and 0.5 ug Anti-Mouse CD223 (65098-1-Ig, Clone: C9B7W) or 0.5 ug rat IgG1 isotype control, and FITC anti-rat IgG1 Antibody at dilution 1:100. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Con-A stimulated mouse splenocytes

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

65098-1-Ig bindet in FC CD223 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Ratte / IgG1, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Murines CD223-Ig-Fusionsprotein
• Vollständiger Name: lymphocyte-activation gene 3
• GenBank-Zugangsnummer: BC120591
• Gene symbol: Lag3
• Gene ID (NCBI): 16768
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide.
• Lagerungsbedingungen: Store at 2-8°C. Stable for one year after shipment.

Hintergrundinformationen

Lymphocyte activation gene 3 protein (LAG-3), also known as CD223, is a type I transmembrane protein belonging to the immunoglobulin superfamily (PMID: 1692078). It is a CD4-related molecule that binds MHC class II with high affinity (PMID: 1692078; 9159144). LAG-3 is expressed on activated T and NK cells. Besides, natural CD4(+)CD25(+) Tregs express LAG-3 upon activation (PMID: 15485628). LAG-3 negatively regulates T-cell function and homeostasis, and contributes to the suppressor activity of Tregs (PMID: 14644131; 15485628).