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CD226 Rekombinanter Antikörper

CD226 Rekombinant Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

230041E12

Kat-Nr. : 82991-3-PBS

Synonyme

DNAM-1, DNAM1, DNAM 1, CD226 molecule, CD226 antigen

Filter:
  • Western Blot (WB) analysis of human peripheral blood platelets using CD226 Recombinant antibody (82991-3-RR)

    WB analysis of human peripheral blood platelets using 82991-3-RR (same clone as 82991-3-PBS)

    human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 82991-3-RR (CD226 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82991-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD226 Recombinant antibody (82991-3-RR)

    IHC staining of human tonsillitis using 82991-3-RR (same clone as 82991-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 82991-3-RR (CD226 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82991-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEL cells using CD226 Recombinant antibody (82991-3-RR)

    IF Staining of HEL using 82991-3-RR (same clone as 82991-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEL cells using CD226 antibody (82991-3-RR, Clone: 230041E12 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82991-3-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

82991-3-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA CD226 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen Rekombinantes Protein
Vollständiger Name CD226 molecule
Berechnetes Molekulargewicht 336 aa, 39 kDa
Beobachtetes Molekulargewicht65 kDa
GenBank-ZugangsnummerBC074787
Gene symbol CD226
Gene ID (NCBI) 10666
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein A purfication
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD226 (DNAM-1) is a ~65 kDa glycoprotein expressed on the surface of NK cells, platelets, monocytes and a subset of T cells. It is a member of the Ig-superfamily containing 2 Ig-like domains of the V-set. CD226 mediates cellular adhesion of platelets and megakaryocytic cells to vascular endothelial cells. The protein also plays a role in megakaryocytic cell maturation. Interactions of CD226 and its ligands, CD155 and CD112, induce NK and T cell-mediated cytotoxicity and cytokine secretion (PMID: 15039383).