CD226 Rekombinanter Antikörper

CD226 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

240673G4

Kat-Nr. : 82991-4-RR

Synonyme

DNAM-1, DNAM1, DNAM 1, CD226 molecule, CD226 antigen

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  WB analysis of human peripheral blood platelets using 82991-4-RR

  human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 82991-4-RR (CD226 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 82991-4-RR

  Biolayer interferometry (BLl) kinetic assays of 82991-4-RR against Human CD226 were performed. The affinity constant is 2.42 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: human peripheral blood platelets

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

82991-4-RR bindet in WB, ELISA CD226 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Fusionsprotein
• Vollständiger Name: CD226 molecule
• Berechnetes Molekulargewicht: 336 aa, 39 kDa
• Beobachtetes Molekulargewicht: 65-70 kDa
• GenBank-Zugangsnummer: BC074787
• Gene symbol: CD226
• Gene ID (NCBI): 10666
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD226 (DNAM-1) is a ~65 kDa glycoprotein expressed on the surface of NK cells, platelets, monocytes and a subset of T cells. It is a member of the Ig-superfamily containing 2 Ig-like domains of the V-set. CD226 mediates cellular adhesion of platelets and megakaryocytic cells to vascular endothelial cells. The protein also plays a role in megakaryocytic cell maturation. Interactions of CD226 and its ligands, CD155 and CD112, induce NK and T cell-mediated cytotoxicity and cytokine secretion (PMID: 15039383).