CD23 Polyklonaler Antikörper

CD23 Polyklonal Antikörper für FC, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, FC,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 18642-1-AP

Synonyme

BLAST 2, CD23, CD23A, CLEC4J, Fc epsilon RII, FCE2, FCER2, IGEBF, Lymphocyte IgE receptor

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Galerie der Validierungsdaten

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  WB analysis of Raji using 18642-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 18642-1-AP (FCER2,CD23 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 18642-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 18642-1-AP (FCER2,CD23 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human tonsillitis using 18642-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 18642-1-AP (FCER2,CD23 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human tonsillitis using 18642-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 18642-1-AP (FCER2,CD23 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0.

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  IHC staining of human tonsillitis using 18642-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 18642-1-AP (FCER2,CD23 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0.

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  IHC staining of human lymphoma using 18642-1-AP

  Immunohistochemical analysis of paraffin-embedded human diffuse small B-cell lymphoma tissue slide using 18642-1-AP (FCER2,CD23 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lymphoma using 18642-1-AP

  Immunohistochemical analysis of paraffin-embedded human diffuse small B-cell lymphoma tissue slide using 18642-1-AP (FCER2,CD23 Antibody) at dilution of 1:50 (under 40x lens).

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  FC experiment of Raji using 18642-1-AP

  1X10^6 Raji cells were stained with 0.2ug FCER2,CD23 antibody (18642-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Raji-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Lymphomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: Raji-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

18642-1-AP bindet in WB, IHC, FC,ELISA CD23 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CD23 fusion protein Ag13348
• Vollständiger Name: Fc fragment of IgE, low affinity II, receptor for (CD23)
• Berechnetes Molekulargewicht: 36 kDa
• Beobachtetes Molekulargewicht: 38-42 kDa
• GenBank-Zugangsnummer: BC014108
• Gene symbol: FCER2
• Gene ID (NCBI): 2208
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD23, also known as low affinity immunoglobulin epsilon Fc receptor, is a transmembrane glycoprotein present on a subpopulation of B lymphocytes in germinal centres, EBV-transformed B-lymphoblastoid cell lines, follicular dendritic cells, and a subpopulation of peripheral blood cells. CD23 has essential roles in the regulation of IgE production and in the differentiation of B-cells.