CD23 Monoklonaler Antikörper

CD23 Monoklonal Antikörper für WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3H6B8

Kat-Nr. : 60208-1-PBS

Synonyme

3H6B8, BLAST 2, BLAST-2, CD23A, CLEC4J

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  WB analysis using 60208-1-Ig (same clone as 60208-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60208-1-Ig (CD23 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60208-1-PBS in a different storage buffer formulation.

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  WB analysis of DC2.4 using 60208-1-Ig (same clone as 60208-1-PBS)

  DC2.4 cells were subjected to SDS PAGE followed by western blot with 60208-1-Ig (CD23 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60208-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 60208-1-Ig (same clone as 60208-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60208-1-Ig (CD23 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60208-1-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 60208-1-Ig (same clone as 60208-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using CD23 antibody (60208-1-Ig, Clone: 3H6B8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60208-1-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 60208-1-Ig (same clone as 60208-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using CD23 antibody (60208-1-Ig, Clone: 3H6B8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60208-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50220-3

  Cytometric bead array standard curve of MP50220-3, CD23,FCER2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60208-2-PBS. Detection antibody: 60208-1-PBS. Standard:Ag0425. Range: 0.391-100 ng/mL.

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  Cytometric bead array standard curve of MP50220-4

  Cytometric bead array standard curve of MP50220-4, CD23,FCER2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60208-1-PBS. Detection antibody: 60208-4-PBS. Standard:Ag0425. Range: 0.391-100 ng/mL.

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Geprüfte Anwendungen

Produktinformation

60208-1-PBS bindet in WB, IHC, IF-P, Cytometric bead array, Indirect ELISA CD23 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CD23 fusion protein Ag0425
• Vollständiger Name: Fc fragment of IgE, low affinity II, receptor for (CD23)
• Berechnetes Molekulargewicht: 321 aa, 36 kDa
• Beobachtetes Molekulargewicht: 36 kDa
• GenBank-Zugangsnummer: BC064417
• Gene symbol: CD23
• Gene ID (NCBI): 2208
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD23, also known as low affinity immunoglobulin epsilon Fc receptor, is a transmembrane glycoprotein present on a subpopulation of B lymphocytes in germinal centres, EBV-transformed B-lymphoblastoid cell lines, follicular dendritic cells, and a subpopulation of peripheral blood cells. CD23 has essential roles in the regulation of IgE production and in the differentiation of B-cells.