CD23 Monoklonaler Antikörper

CD23 Monoklonal Antikörper für WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

4C7C9

Kat-Nr. : 60208-2-PBS

Synonyme

C-type lectin domain family 4 member J, CLEC4J, CD23A, BLAST-2, BLAST 2

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  WB analysis of Raji using 60208-2-Ig (same clone as 60208-2-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 60208-2-Ig (CD23 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60208-2-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 60208-2-Ig (same clone as 60208-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60208-2-Ig (CD23 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60208-2-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 60208-2-Ig (same clone as 60208-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60208-2-Ig (CD23 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60208-2-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 60208-2-Ig (same clone as 60208-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60208-2-Ig (CD23 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60208-2-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 60208-2-Ig (same clone as 60208-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human diffuse small B-cell lymphoma using 60208-2-Ig(CD23 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60208-2-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 60208-2-Ig (same clone as 60208-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human diffuse small B-cell lymphoma using 60208-2-Ig(CD23 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60208-2-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 60208-2-Ig (same clone as 60208-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using CD23 antibody (60208-2-Ig, Clone: 4C7C9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60208-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50220-1

  Cytometric bead array standard curve of MP50220-1, CD23,FCER2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60208-2-PBS. Detection antibody: 60208-3-PBS. Standard:Ag0425. Range: 0.781-100 ng/mL.

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  Cytometric bead array standard curve of MP50220-2

  Cytometric bead array standard curve of MP50220-2, CD23,FCER2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60208-2-PBS. Detection antibody: 60208-4-PBS. Standard:Ag0425. Range: 0.781-100 ng/mL.

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  Cytometric bead array standard curve of MP50220-3

  Cytometric bead array standard curve of MP50220-3, CD23,FCER2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60208-2-PBS. Detection antibody: 60208-1-PBS. Standard:Ag0425. Range: 0.391-100 ng/mL.

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Geprüfte Anwendungen

Produktinformation

60208-2-PBS bindet in WB, IHC, IF-P, Cytometric bead array, Indirect ELISA CD23 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CD23 fusion protein Ag0425
• Vollständiger Name: Fc fragment of IgE, low affinity II, receptor for (CD23)
• Berechnetes Molekulargewicht: 321 aa, 36 kDa
• Beobachtetes Molekulargewicht: 40 kDa
• GenBank-Zugangsnummer: BC064417
• Gene symbol: CD23
• Gene ID (NCBI): 2208
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD23, also known as low affinity immunoglobulin epsilon Fc receptor, is a transmembrane glycoprotein present on a subpopulation of B lymphocytes in germinal centres, EBV-transformed B-lymphoblastoid cell lines, follicular dendritic cells, and a subpopulation of peripheral blood cells. CD23 has essential roles in the regulation of IgE production and in the differentiation of B-cells.