CD23 Rekombinanter Antikörper

CD23 Rekombinant Antikörper für WB, IHC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241269B10

Kat-Nr. : 84024-2-PBS

Synonyme

241269B10, BLAST 2, BLAST-2, CD23A, CLEC4J

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  WB analysis using 84024-2-RR (same clone as 84024-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84024-2-RR (CD23,FCER2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84024-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 84024-2-RR (same clone as 84024-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84024-2-RR (CD23 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84024-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 84024-2-RR (same clone as 84024-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84024-2-RR (CD23 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84024-2-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 84024-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83996-4-RR against Human CD23 were performed. The affinity constant is 3.66 nM.

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Geprüfte Anwendungen

Produktinformation

84024-2-PBS bindet in WB, IHC, Cytometric bead array, Indirect ELISA CD23 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: Fc fragment of IgE, low affinity II, receptor for (CD23)
• Berechnetes Molekulargewicht: 321 aa, 36 kDa
• Beobachtetes Molekulargewicht: 36 kDa
• GenBank-Zugangsnummer: BC064417
• Gene symbol: CD23
• Gene ID (NCBI): 2208
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD23, also known as low-affinity immunoglobulin epsilon Fc receptor, is a transmembrane glycoprotein present on a subpopulation of B lymphocytes in germinal centers, EBV-transformed B-lymphoblastoid cell lines, follicular dendritic cells, and a subpopulation of peripheral blood cells. CD23 has essential roles in the regulation of IgE production and in the differentiation of B-cells