CD23 Rekombinanter Antikörper

CD23 Rekombinant Antikörper für Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

241269C8

Kat-Nr. : 84024-3-PBS

Synonyme

BLAST 2, BLAST-2, CD23A, CLEC4J, C-type lectin domain family 4 member J

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  Sandwich ELISA standard curve of MP00958-3

  Sandwich ELISA standard curve of MP00958-3, Human CD23 Recombinant Matched Antibody Pair - PBS only. 84024-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard RP02343. 84024-1-PBS was HRP conjugated as the detection antibody. Range: 7.81-500 pg/mL

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  Sample test of MP00958-3

  Serum of sixteen individual healthy human donors was measured. The human CD23 concentration of detected samples was determined to be 651.0 pg/mL with a range of 259.5 - 1,165.0 pg/mL.

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  Sample test of MP00958-3

  Human peripheral blood mononuclear cells (PBMC) were cultured unstimulated or stimulated with 10 μg/mL PHA for 3 days. The mean CD23 concentration was determined to be 16.3 pg/mL in unstimulated PBMC supernatant, 444.73 pg/mL in PHA stimulated PBMC supernatant. Raji human Burkitt’s lymphoma cells were cultured in RPMI supplemented with 10% fetal bovine serum, 2mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. The mean CD23 concentration was determined to be 742.6 pg/mL Raji cell culture supernatants.

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Geprüfte Anwendungen

Produktinformation

84024-3-PBS bindet in Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test CD23 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: Fc fragment of IgE, low affinity II, receptor for (CD23)
• Berechnetes Molekulargewicht: 321 aa, 36 kDa
• GenBank-Zugangsnummer: BC064417
• Gene symbol: CD23
• Gene ID (NCBI): 2208
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.