CD23 Rekombinanter Antikörper

CD23 Rekombinant Antikörper für WB, IHC, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, IHC, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

241385C9

Kat-Nr. : 84212-3-PBS

Synonyme

241385C9, Fc-epsilon-RII, Fcer2, Fcer2a, Low affinity immunoglobulin epsilon Fc receptor

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  WB analysis of mouse spleen using 84212-3-RR (same clone as 84212-3-PBS)

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 84212-3-RR (CD23/Fc epsilon RII antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84212-3-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01134-2

  Sandwich ELISA standard curve of MP01134-2, Mouse CD23 Recombinant Matched Antibody Pair - PBS only. 84212-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1295. 84212-3-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL.

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  Sample test of MP01134-2

  Serum of six mouse was measured. The CD23 concentration of detected samples was determined to be 2624.00 pg/mL with a range of 1279.00 - 7445.00 pg/mL.

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  IHC staining of mouse spleen using 84212-3-RR (same clone as 84212-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84212-3-RR (CD23/Fc epsilon RII antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84212-3-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 84212-3-RR (same clone as 84212-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84212-3-RR (CD23/Fc epsilon RII antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84212-3-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84212-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84212-3-RR against Mouse CD23/Fc epsilon RII were performed. The affinity constant is 0.168 nM.

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Geprüfte Anwendungen

Produktinformation

84212-3-PBS bindet in WB, IHC, Sandwich ELISA, Indirect ELISA, Sample test CD23 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD23 fusion protein Eg1295
• Vollständiger Name: Fc receptor, IgE, low affinity II, alpha polypeptide
• Berechnetes Molekulargewicht: 38kDa
• Beobachtetes Molekulargewicht: 46 kDa
• GenBank-Zugangsnummer: EDL21948.1
• Gene symbol: Fcer2a
• Gene ID (NCBI): 14128
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD23/Fc ε RII) is a low-affinity receptor for immunoglobulin E (IgE) and CR2/CD21, involved in B cell differentiation and IgE regulation. Two forms of Fc ε RII (Fc ε RIIa and Fc ε RIIb) were found to exist, which differ in structure only in the N-terminal cytoplasmic region, and Fc ε RIIa and Fc ε RIIb play roles in effector cells of B-cell and IgE-mediated immunity, respectively. On B cells, initiates IgE-dependent antigen uptake and presentation to T cells. On macrophages, after IgE binding and antigen cross-linking, intracellular killing of parasites is induced by activation of the L-arginine-nitric oxide pathway.