Glycophorin A/CD235a Monoklonaler Antikörper

Glycophorin A/CD235a Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / IgG2b, kappa

Getestete Reaktivität

human

Anwendung

FC

Konjugation

PE Fluorescent Dye

CloneNo.

HIR2

Kat-Nr. : PE-65174

Synonyme

Glycophorin A, CD235a, GpMiIII, GPErik, GPA

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  FC experiment of human red blood cells using PE-65174

  10 ul human whole blood was surface stained with 5 ul PE Anti-Human CD235a (HIR2) (PE-65174) or PE Mouse IgG2b Isotype Control. Cells were not fixed. Red blood cells were gated.

• 

  FC experiment of human red blood cells using PE-65174

  10 ul human whole blood was surface stained with 5 ul PE Anti-Human CD235a (HIR2) (PE-65174) or PE Mouse IgG2b Isotype Control. Cells were not fixed. Red blood cells were gated.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane Erythrozyten

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

PE-65174 bindet in FC Glycophorin A/CD235a und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Synthetisches Peptid (Human, N-Terminus)
• Vollständiger Name: glycophorin A (MNS blood group)
• Berechnetes Molekulargewicht: 150 aa, 16 kDa
• GenBank-Zugangsnummer: BC005319
• Gene symbol: Glycophorin A
• Gene ID (NCBI): 2993
• Konjugation: PE Fluorescent Dye
• Excitation/Emission maxima wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Reinigungsmethode: Der gereinigte Antikörper ist mit R-Phycoerythrin (PE) unter optimalen Bedingungen konjugiert. Das Konjugat wird mit Größenausschluss-Chromatographie gereinigt.
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

Glycophorin A (GYPA) is the major transmembrane sialoglycoprotein in erythrocytes. It is a dimeric type I transmembrane protein carrying 15 closely clustered O-linked tetrasaccharides capped with sialic acid/N-acetylneuraminic acid (Neu5Ac). This 36 kDa protein represents the major sialoglycoprotein of the red blood cell membrane displaying about one million copies per cell. (PMID: 9490702)