CD24 Polyklonaler Antikörper

CD24 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

13

Kat-Nr. : 18330-1-AP

Synonyme

CD24, CD24 molecule, CD24A, Signal transducer CD24

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Galerie der Validierungsdaten

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  WB analysis of MCF-7 using 18330-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 18330-1-AP (CD24 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 18330-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 18330-1-AP (CD24 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 18330-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 18330-1-AP (CD24 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, Mausmilzgewebe, Raji-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 10 publications below
• IF: See 1 publications below

Produktinformation

18330-1-AP bindet in WB, IF, ELISA CD24 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CD24 fusion protein Ag11679
• Vollständiger Name: CD24 molecule
• Berechnetes Molekulargewicht: 8 kDa
• Beobachtetes Molekulargewicht: 30-70 kDa
• GenBank-Zugangsnummer: BC007674
• Gene symbol: CD24
• Gene ID (NCBI): 100133941
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD24 is a cell surface signal transducing molecule, which is also known as a cluster of differentiation 24 or heat stable antigen CD24 (HSA) (PMID: 7959762). It is a sialoglycoprotein that is anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) link. Due to its cell surface localization, it contributes to a wide range of downstream signaling networks and has been shown to play crucial roles during neural development (PMID: 27993646) and the differentiation of various cell types. For example, it promotes antigen-dependent proliferation of B-cells, while preventing their terminal differentiation into antibody-producing cells (PMID: 11313396). CD24 may also play a role in apoptosis regulation, as cross-linking of this protein on the surface of neutrophils induces cell death, which seems to be defective in sepsis (PMID: 24501201). In addition, CD24 has been linked to negative regulation of the immune responses, particularly to danger-associated molecular patterns (DAMPs) (PMID: 24996822). 

What is the molecular weight of CD24?

According to NCBI, there are two known isoforms of CD24; the shorter 80 aa (8 kDa) isoform 1, which is considered a canonical sequence that has been experimentally validated, and a longer 120 aa (12,9 kDa) protein (PMIDs: 16344560, 15489334). Isoform 1 is further processed into a mature 32 aa form. Apart from these, 3 potential computationally mapped isoforms have been suggested. The CD24 gene locus is localized on chromosome 6, while other non-transcribed loci have been found on chromosomes 1, 15, 20, and Y (PMID: 7959762). 

What is the subcellular localization of CD24?

It is a cell surface protein that can act as an adhesion molecule. 

What is the tissue specificity of CD24?

As a rule, CD24 tends to be expressed at higher levels in progenitor cells and metabolically active cells and to a lesser extent in terminally differentiated cells (PMID: 25613900). CD24 is specifically expressed in a number of B-cell lines, granulocytes, and differentiating neuroblasts (PMID: 8753773, 12447971). The expression is lost when primary B-cells are induced to differentiate in antibody-forming cells. CD24 expression has also been linked with various malignancies: epithelial neoplasms (PMID:16164042), erythroleukemia cell and small cell lung carcinoma cell lines. For certain cancers it is considered a prognostic marker, usually associated with poor patient prognosis, e.g., colorectal cancer (PMID: 16166435) and ovarian cancer (PMID: 2368195). 

What is the involvement of CD24 in disease?

As mentioned before it is considered a prognostic marker in various malignancies. However, it is also clearly associated with multiple sclerosis (MS). Indeed, a single nucleotide polymorphism V57A has been associated with an over 2-fold increase in the susceptibility to MS and has been generally linked to a quicker progression of the disease in the general population (PMID: 14657362). 

What are the main roles of CD24?

Considering its expression patterns, it is clear why CD24 plays the most important roles in immune cells. It can be used as a marker for the differentiation of hematopoietic and neuronal cells, in addition to tissue and tumor stem cells (PMID: 12447971). CD24 has also been found to mediate apoptosis in precursor-B acute lymphoblastic leukemia cell lines in the pro-B and pre-B stages accompanying activation of multiple caspases (PMID: 12496407). It has also been shown to play a role in autoimmune responses, as CD24-deficient mice are resistant to autoimmune encephalomyelitis (PMID: 10791997). 

Publikationen

Species	Application	Title
human	WB	Cancer Lett; Cancer stem-like cell characteristics induced by EB virus-encoded LMP1 contribute to radioresistance in nasopharyngeal carcinoma by suppressing the p53-mediated apoptosis pathway.; Authors - Chang-Fu Yang
human	WB	Cancer Cell Int; SOCS6 promotes radiosensitivity and decreases cancer cell stemness in esophageal squamous cell carcinoma by regulating c-Kit ubiquitylation.; Authors - Xuanzi Sun
human	WB	Biomolecules; COL11A1-Driven Epithelial-Mesenchymal Transition and Stemness of Pancreatic Cancer Cells Induce Cell Migration and Invasion by Modulating the AKT/GSK-3β/Snail Pathway.; Authors - Hui Wang
		Int J Oncol; PMID: 29956726; Authors - Pengfei Su
human	WB	Acta Biochim Biophys Sin (Shanghai); miR-345 inhibits migration and stem-like cell phenotype in gastric cancer via inactivation of Rac1 by targeting EPS8.; Authors - Jieyun Zhang
human	WB	J Oncol; Acidic Tumor Microenvironment Promotes Pancreatic Cancer through miR-451a/MEF2D Axis.; Authors - Jingyong Xu

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