CD244 Rekombinanter Antikörper

CD244 Rekombinant Antikörper für WB, IHC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241260C6

Kat-Nr. : 84086-1-PBS

Synonyme

SLAMF4, 241260C6, 2B4, h2B4, NAIL

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  WB analysis using 84086-1-RR (same clone as 84086-1-PBS)

  NK-92 cells were subjected to SDS PAGE followed by western blot with 84086-1-RR (CD244 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84086-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 84086-1-RR (same clone as 84086-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84086-1-RR (CD244 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84086-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 84086-1-RR (same clone as 84086-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84086-1-RR (CD244 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84086-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP01008-3

  Cytometric bead array standard curve of MP01008-3, CD244 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84086-2-PBS. Detection antibody: 84086-1-PBS. Standard: Eg32087. Range: 0.625-80 ng/mL.

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  Affinity and Kinetic Characterization of 84086-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84086-1-RR against Human CD244 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

84086-1-PBS bindet in WB, IHC, Cytometric bead array, Indirect ELISA CD244 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD244 fusion protein Eg32087
• Vollständiger Name: CD244 molecule, natural killer cell receptor 2B4
• Berechnetes Molekulargewicht: 365 aa, 41 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC053985
• Gene symbol: CD244
• Gene ID (NCBI): 51744
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD244, also known as SLAMF4 or 2B4, is a type I transmembrane glycoprotein belonging to the signaling lymphocyte activation molecule (SLAM) family. It consists of an extracellular segment with two immunoglobulin (Ig)-like domains, a transmembrane region, and a cytoplasmic domain containing tyrosine-based motifs (PMID: 9841922; 30546369). CD244 is present on natural killer (NK) cells, γδ T cells, a subset of CD8+ T cells, monocytes, basophils, dendritic cells, and myeloid-derived suppressor cells (MDSCs) (PMID: 30546369). It binds to CD48 with high affinity and transmits stimulatory or inhibitory signals that regulate immune function (PMID: 9841922; 18523281).