CD244 Rekombinanter Antikörper

CD244 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

CoraLite® Plus 405 Fluorescent Dye

CloneNo.

241260G10

Kat-Nr. : CL405-98133

Synonyme

SLAMF4, 241260G10, 2B4, h2B4, NAIL

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  FC experiment of human PBMCs using CL405-98133

  1x10^6 human PBMCs were surface stained with CoraLite® Plus 488 Anti-Human CD3, and 5 ul CoraLite® Plus 405 Anti-Human CD244 Rabbit RecAb (CL405-98133, Clone: 241260G10) or CoraLite® Plus 405 Rabbit IgG Isotype Control RecAb (CL405-98136, Clone: 240953C9). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human PBMCs using CL405-98133

  1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 405 Anti-Human CD244 Rabbit RecAb (CL405-98133, Clone:241260G10) (red), or CoraLite® Plus 405 Rabbit IgG Isotype Control RecAb (CL405-98136, Clone: 240953C9) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

CL405-98133 bindet in FC CD244 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD244 fusion protein Eg32087
• Vollständiger Name: CD244 molecule, natural killer cell receptor 2B4
• Berechnetes Molekulargewicht: 365 aa, 41 kDa
• GenBank-Zugangsnummer: BC053985
• Gene symbol: CD244
• Gene ID (NCBI): 51744
• Konjugation: CoraLite® Plus 405 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 399 nm / 422 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD244, also known as SLAMF4 or 2B4, is a type I transmembrane glycoprotein belonging to the signaling lymphocyte activation molecule (SLAM) family. It consists of an extracellular segment with two immunoglobulin (Ig)-like domains, a transmembrane region, and a cytoplasmic domain containing tyrosine-based motifs (PMID: 9841922; 30546369). CD244 is present on natural killer (NK) cells, γδ T cells, a subset of CD8+ T cells, monocytes, basophils, dendritic cells, and myeloid-derived suppressor cells (MDSCs) (PMID: 30546369). It binds to CD48 with high affinity and transmits stimulatory or inhibitory signals that regulate immune function (PMID: 9841922; 18523281).