CD25/IL-2R alpha Rekombinanter Antikörper

CD25/IL-2R alpha Rekombinant Antikörper für WB, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus, Ratte

Anwendung

WB, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

250670H3

Kat-Nr. : 86213-1-PBS

Synonyme

CD25, IL-2 receptor subunit alpha, IL-2R subunit alpha, Il2ra, IL-2-RA

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  WB analysis using 86213-1-RR (same clone as 86213-1-PBS)

  Recombinant protein were subjected to SDS PAGE followed by western blot with 86213-1-RR (CD25/IL-2R alpha antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86213-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 86213-1-RR (same clone as 86213-1-PBS)

  CTLL-2 cells were subjected to SDS PAGE followed by western blot with 86213-1-RR (CD25/IL-2R alpha antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86213-1-PBS in a different storage buffer formulation.

• 

  Sandwich ELISA standard curve of MP02447-1

  Sandwich ELISA standard curve of MP02447-1, Rat CD25/IL-2RA Recombinant Matched Antibody Pair - PBS only. 86213-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1453. 86213-2-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

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  Sample test of MP02447-1

  Serum of sixteen rats was measured. The CD25/IL-2RA concentration of detected samples was determined to be 0.89 ng/mL with a range of 0.44-5.27 ng/mL.

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Geprüfte Anwendungen

Produktinformation

86213-1-PBS bindet in WB, Sandwich ELISA, Indirect ELISA, Sample test CD25/IL-2R alpha und zeigt Reaktivität mit Maus, Ratten

• Getestete Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD25/IL-2R alpha fusion protein Eg1453
• Vollständiger Name: interleukin 2 receptor, alpha
• Berechnetes Molekulargewicht: 31kDa
• Beobachtetes Molekulargewicht: 55-60 kDa
• GenBank-Zugangsnummer: NM_013163.1
• Gene symbol: Il2ra
• Gene ID (NCBI): 25704
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Proliferation of T lymphocytes is triggered by the interaction of IL-2 with its specific receptor following T lymphocyte activation. The receptor for IL-2 has three forms, generated by different combinations of three different proteins, the alpha chain (IL-2R alpha), the beta chain (IL-2R beta), and the gamma chain (IL-2R gamma) (PMID: 8476561). IL-2R alpha (also known as CD25) is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes. CD25 is also found on natural CD4+ Foxp3+ Treg cells (PMID: 22585674).