CD25/IL-2RA Rekombinanter Antikörper

CD25/IL-2RA Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

240906E11

Kat-Nr. : 98469-1-RR

Synonyme

CD25, IL 2 RA, IL 2 receptor subunit alpha, IL 2R subunit alpha, IL2 RA

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  FC experiment of human PBMCs using 98469-1-RR

  1x10^6 untreated or PHA-treated human PBMCs were surface stained with 0.25 ug Anti-Human CD25/IL-2RA Rabbit RecAb (98469-1-RR, Clone: 240906E11) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD4. Cells were not fixed.

• 

  FC experiment of human PBMCs using 98469-1-RR

  1x10^6 PHA-treated human PBMCs were surface stained with 0.25 ug Anti-Human CD25/IL-2RA Rabbit RecAb (98469-1-RR, Clone: 240906E11) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated human PBMCs were surface stained with 0.25 ug Anti-Human CD25/IL-2RA Rabbit RecAb (98469-1-RR, Clone: 240906E11) (black, dashed) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

• 

  Affinity and Kinetic Characterization of 98469-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98469-1-RR against Human CD25/IL-2RA were performed. The affinity constant is 0.387 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: PHA-treated human PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98469-1-RR bindet in FC CD25/IL-2RA und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD25/IL-2RA fusion protein Eg0408
• Vollständiger Name: interleukin 2 receptor, alpha
• Berechnetes Molekulargewicht: 272 aa, 31 kDa
• GenBank-Zugangsnummer: BC156627
• Gene symbol: IL2RA
• Gene ID (NCBI): 3559
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

Proliferation of T lymphocytes is triggered by the interaction of IL-2 with its specific receptor following T lymphocyte activation. The receptor for IL-2 has three forms, generated by different combinations of three different proteins, the alpha chain (IL-2R alpha), the beta chain (IL-2R beta), and the gamma chain (IL-2R gamma) (PMID: 8476561). IL-2R alpha (also known as CD25) is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes. CD25 is also found on natural CD4+Foxp3+ Treg cells and a subset of human CD4+ memory T cells (PMID: 22585674).