CD25/IL-2RA Rekombinanter Antikörper

CD25/IL-2RA Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

APC Fluorescent Dye

CloneNo.

242494H5

Kat-Nr. : APC-FcA98288

Synonyme

CD25, IL-2 receptor subunit alpha, Il2r, IL-2R subunit alpha, Il2ra

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  FC experiment of mouse splenocytes using APC-FcA98288

  1x10^6 untreated or anti-CD3/CD28 treated mouse splenocytes were surface stained with 0.1 ug APC Anti-Mouse CD25/IL-2RA Rabbit RecAb (APC-FcA98288, Clone: 242494H5). Cells were co-stained with FcZero-rAb™ PE Anti-Mouse CD4 (RM4-4). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Anti-CD3/CD28 treated mouse splenocytes

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

APC-FcA98288 bindet in FC CD25/IL-2RA und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD25/IL-2RA fusion protein Eg3087
• Vollständiger Name: interleukin 2 receptor, alpha chain
• Berechnetes Molekulargewicht: 31 kDa
• GenBank-Zugangsnummer: NM_008367.3
• Gene symbol: CD25
• Gene ID (NCBI): 16184
• Konjugation: APC Fluorescent Dye
• Excitation/Emission maxima wavelengths: 650 nm / 660 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

Proliferation of T lymphocytes is triggered by the interaction of IL-2 with its specific receptor following T lymphocyte activation. The receptor for IL-2 has three forms, generated by different combinations of three different proteins, the alpha chain (IL-2R alpha), the beta chain (IL-2R beta), and the gamma chain (IL-2R gamma) (PMID: 8476561). IL-2R alpha (also known as CD25) is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes. CD25 is also found on natural CD4+Foxp3+ Treg cells and a subset of human CD4+ memory T cells (PMID: 22585674).