CD25/IL-2RA Rekombinanter Antikörper

CD25/IL-2RA Rekombinant Antikörper für FC

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Affe, human, nicht-humane Primaten

Anwendung

FC

Konjugation

PE-Cyanine5.5 Fluorescent Dye

CloneNo.

BC96

Kat-Nr. : PY5P5-65620

Synonyme

CD25, BC96, IL 2 RA, IL 2 receptor subunit alpha, IL 2R subunit alpha

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  FC experiment of human PBMCs using PY5P5-65620

  1x10^6 untreated or PHA-treated human PBMCs were surface stained with 5 ul PE-Cyanine5.5 Anti-Human CD25/IL-2RA (BC96) Mouse IgG2a RecAb (PY5P5-65620, Clone: BC96) and 5 ul CoraLite® Plus 488 Anti-Human CD4 Rabbit Recombinant Antibody (CL488-98042, Clone: 240427E12). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: PHA treated human PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

PY5P5-65620 bindet in FC CD25/IL-2RA und zeigt Reaktivität mit Affe, human, nicht-humane Primaten

• Getestete Reaktivität: Affe, human, nicht-humane Primaten
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: k.A.
• Vollständiger Name: interleukin 2 receptor, alpha
• Berechnetes Molekulargewicht: 272 aa, 31 kDa
• GenBank-Zugangsnummer: BC156627
• Gene symbol: IL2RA
• Gene ID (NCBI): 3559
• Konjugation: PE-Cyanine5.5 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 488 nm, 561 nm / 692 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

Proliferation of T lymphocytes is triggered by the interaction of IL-2 with its specific receptor following T lymphocyte activation. The receptor for IL-2 has three forms, generated by different combinations of three different proteins, the alpha chain (IL-2R alpha), the beta chain (IL-2R beta), and the gamma chain (IL-2R gamma) (PMID: 8476561). IL-2R alpha (also known as CD25) is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes. CD25 is also found on natural CD4+Foxp3+ Treg cells and a subset of human CD4+ memory T cells (PMID: 22585674).