CD27 Rekombinanter Antikörper

CD27 Rekombinant Antikörper für FC

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

FC

Konjugation

Biotin

CloneNo.

O323

Kat-Nr. : Biotin-65627

Synonyme

CD27 antigen, CD27 molecule, CD27L receptor, S152, T cell activation antigen CD27

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  FC experiment of human PBMCs using Biotin-65627

  1x10^6 human PBMCs were surface stained with 0.25 ug Biotin Anti-Human CD27 (O323) Mouse IgG2a RecAb (Biotin-65627, Clone: O323) and CoraLite®488-conjugated streptavidin, or unstained. Cells were co-stained with APC Anti-Human CD3. Cells were not fixed.

• 

  FC experiment of human PBMCs using Biotin-65627

  1x10^6 human PBMCs were surface stained with 0.25 ug Biotin Anti-Human CD27 (O323) Mouse IgG2a RecAb (Biotin-65627, Clone: O323) (red) and CoraLite®488-conjugated streptavidin, or unstained (blue). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

Biotin-65627 bindet in FC CD27 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: k.A.
• Vollständiger Name: CD27 molecule
• Berechnetes Molekulargewicht: 29 kDa
• GenBank-Zugangsnummer: BC012160
• Gene symbol: CD27
• Gene ID (NCBI): 939
• Konjugation: Biotin
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD27 (also known as TNFRSF7) is a type I glycoprotein expressed on some B cells and the majority of T cells, and is a member of the tumor necrosis factor (TNF) receptor family. CD27 is required for generation and long-term maintenance of T cell immunity (PMID: 11062504). It is a receptor for CD70 (CD27L). Ligation of CD27 by CD70 induces strong ubiquitination of TRAF and the activation of both canonical and non-canonical NF-kappaB pathways, as well as the JNK pathway (PMID: 19426224). CD27 may also play a role in apoptosis through association with SIVA1.