CD27 Rekombinanter Antikörper

CD27 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

CoraLite®700 Fluorescent Dye

CloneNo.

241378E11

Kat-Nr. : CL700-98160

Synonyme

241378E11, CD27 antigen, CD27L receptor, T-cell activation antigen CD27, Tnfrsf7

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  FC experiment of mouse splenocytes using CL700-98160

  1x10^6 mouse splenocytes were surface stained with FITC Plus Anti-Mouse CD3 (17A2) (FITC-65077, Clone: 17A2), and 0.25 ug CoraLite®700 Anti-Mouse CD27 Rabbit RecAb (CL700-98160, Clone: 241378E11) or unstained. Cells were not fixed. Lymphocytes were gated.

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  FC experiment of mouse splenocytes using CL700-98160

  1x10^6 mouse splenocytes were surface stained with 0.25 ug CoraLite®700 Anti-Mouse CD27 Rabbit RecAb (CL700-98160, Clone: 241378E11) (red) or unstained (blue). Cells were not fixed. Lymphocytes were gated.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Maus-Splenozyten

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL700-98160 bindet in FC CD27 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD27 fusion protein Eg1311
• Vollständiger Name: CD27 antigen
• Berechnetes Molekulargewicht: 28kDa
• GenBank-Zugangsnummer: NM_001033126.2
• Gene symbol: CD27
• Gene ID (NCBI): 21940
• Konjugation: CoraLite®700 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 702 nm / 723 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD27 (also known as TNFRSF7) is a type I glycoprotein expressed on some B cells and the majority of T cells, and is a member of the tumor necrosis factor (TNF) receptor family. CD27 is required for generation and long-term maintenance of T cell immunity (PMID: 11062504). It is a receptor for CD70 (CD27L). Ligation of CD27 by CD70 induces strong ubiquitination of TRAF and the activation of both canonical and non-canonical NF-kappaB pathways, as well as the JNK pathway (PMID: 19426224). CD27 may also play a role in apoptosis through association with SIVA1.