CD27 Rekombinanter Antikörper

CD27 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

PE-Cyanine7 Fluorescent Dye

CloneNo.

242731G8

Kat-Nr. : PY7-98346-3

Synonyme

242731G8, CD27 antigen, CD27 molecule, CD27L receptor, S152

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  FC experiment of human PBMCs using PY7-98346-3

  1x10^6 human PBMCs were surface stained with CoraLite® Plus 488 Anti-Human CD3, and 5 ul PE-Cyanine7 Anti-Human CD27 Rabbit RecAb (PY7-98346-3, Clone: 242731G8) or unstained. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using PY7-98346-3

  1x10^6 human PBMCs were surface stained with 5 ul PE-Cyanine7 Anti-Human CD27 Rabbit RecAb (PY7-98346-3, Clone:242731G8) (red), or unstained (blue). Cells were not fixed. Lymphocytes were gated.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

PY7-98346-3 bindet in FC CD27 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD27 fusion protein Eg1644
• Vollständiger Name: CD27 molecule
• Berechnetes Molekulargewicht: 29 kDa
• GenBank-Zugangsnummer: BC012160
• Gene symbol: CD27
• Gene ID (NCBI): 939
• Konjugation: PE-Cyanine7 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 450-500 nm / 778 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD27 (also known as TNFRSF7) is a type I glycoprotein expressed on some B cells and the majority of T cells, and is a member of the tumor necrosis factor (TNF) receptor family. CD27 is required for generation and long-term maintenance of T cell immunity (PMID: 11062504). It is a receptor for CD70 (CD27L). Ligation of CD27 by CD70 induces strong ubiquitination of TRAF and the activation of both canonical and non-canonical NF-kappaB pathways, as well as the JNK pathway (PMID: 19426224). CD27 may also play a role in apoptosis through association with SIVA1.