PD-L1/CD274 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PD-L1/CD274 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen(210)

Kat-Nr. : 17952-1-AP

Synonyme

CD274, PD-L1, B7 H1, B7-H1, hPD-L1

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  WB analysis using 17952-1-AP

  Untreated and IFN gamma treated A549 cells were subjected to SDS PAGE followed by western blot with 17952-1-AP (PD-L1/CD274 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 17952-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 17952-1-AP (PD-L1/CD274 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 17952-1-AP

  Rat kidney tissue was subjected to SDS PAGE followed by western blot with 17952-1-AP (PD-L1/CD274 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 17952-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 17952-1-AP (PD-L1/CD274 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 17952-1-AP

  WB result of PD-L1 antibody (17952-1-AP, 1:500) with si-Control and si-PD-L1 transfected HepG2 and HeLa cells.

• 

  WB analysis using 17952-1-AP

  Untreated and IFN gamma treated A375 cells were subjected to SDS PAGE followed by western blot with 17952-1-AP (PD-L1/CD274 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 17952-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 17952-1-AP (PD-L1/CD274 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 17952-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17952-1-AP (PD-L1/CD274 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 17952-1-AP

  IP result of anti-PD-L1/CD274 (IP:17952-1-AP, 4ug; Detection:66248-1-Ig 1:1000) with mouse heart tissue lysate 4000ug.

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  IHC staining of human tonsillitis using 17952-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17952-1-AP (PD-L1/CD274 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 17952-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17952-1-AP (PD-L1/CD274 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 17952-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 17952-1-AP (PD-L1 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human stomach cancer using 17952-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 17952-1-AP (PD-L1 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse heart using 17952-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 17952-1-AP (PD-L1/CD274 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of mouse heart using 17952-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 17952-1-AP (PD-L1/CD274 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HEK-293 using 17952-1-AP

  Immunofluorescent analysis of HEK-293 cells using 17952-1-AP (PD-L1/CD274 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Plazenta-Gewebe, A375-Zellen, HepG2-Zellen, mit IFN-gamma behandelte A549-Zellen, K-562-Zellen, Mausherzgewebe, Maus-Skelettmuskelgewebe, Rattennierengewebe
• Erfolgreiche IP: Mausherzgewebe
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Magenkrebsgewebe, Mausherzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 8 publications below
• WB: See 112 publications below
• IHC: See 108 publications below
• IF: See 42 publications below
• IP: See 1 publications below
• CoIP: See 3 publications below
• ChIP: See 1 publications below

Produktinformation

17952-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA PD-L1/CD274 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PD-L1/CD274 fusion protein Ag12432
• Vollständiger Name: CD274 molecule
• Berechnetes Molekulargewicht: 290 aa, 33 kDa
• Beobachtetes Molekulargewicht: 45-56 kDa, 65-70 kDa
• GenBank-Zugangsnummer: BC074984
• Gene symbol: PD-L1
• Gene ID (NCBI): 29126
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PD-L1, also known as CD274 or B7H1, stands for programmed cell death ligand 1. It is a type I transmembrane protein that is thought to repress immune responses by binding to its receptor (PD1), thus inhibiting T-cell activation, proliferation, and cytokine production. It contains V-like and C-like immunoglobulin domains. PD-L1 expression is regulated by various cytokines, such as TNF-α or LPS (ISSN: 1848-7718). Increased expression of this protein in certain types of cancers, e.g., renal cell carcinoma or colon cancer, correlates with poor prognosis. 

What is the molecular weight of PD-L1? 

Depending on the isoform, the calculated molecular weight of the protein varies between 20 and 33 kDa (176-290 aa). 

What are the isoforms of PD-L1? 

According to NCBI, three different isoforms have been identified. There are significant differences in the untranslated and protein coding regions. 

What is the subcellular localization and tissue specificity of PD-L1? 

It is predicted to localize in the plasma membrane of various cell types, with a particularly high expression in placental trophoblast and subsets of immune cells. High levels of PD-L1 protein have also been detected in lung and colon tissues. 

What is the function of PD-L1 in immune responses? 

PD-L1 is critical for the induction and maintenance of immune self-tolerance during infection or inflammation in normal tissues. The interaction of PD-L1 and its receptors is responsible for preventing auto-immune phenotypes and balancing the overall immune response in situations such as pregnancy or tissue allografts. The interaction between PD-L1 and PD-1 or B7.1 starts an inhibitory signaling cascade, which results in the decreased proliferation of antigen-specific T-cells and increased survival of regulatory T-cells (PMID: 15240681). 

How can PD-L1's implication in cancer be used as a drug target? 

In certain tumors, high expression of PD-L1 serves as a stop-sign to inhibit the recognition of cancer cells by T-cells (PMID: 23087408). The interaction between PD-L1 and its receptors (PD1 and B7.1) is a mechanism for the tumor to evade the host immune response (PMID: 29357948). Several mAbs have been developed to target that interaction and thus prevent the inactivation of cytotoxic T-cells by the tumor (PMIDs: 23890059, 18173375).

Publikationen

Species	Application	Title
human	WB	Nat Biomed Eng; Inhibiting PD-L1 palmitoylation enhances T-cell immune responses against tumours.; Authors - Han Yao
human	IHC	Nat Commun; Pharmaceutical targeting of OTUB2 sensitizes tumors to cytotoxic T cells via degradation of PD-L1; Authors - Wenfeng Ren
human	WB	Adv Sci (Weinh); BiTE-Secreting CAR-γδT as a Dual Targeting Strategy for the Treatment of Solid Tumors; Authors - Shi-Wei Huang
mouse,human	IHC,IF	Acta Pharm Sin B; Whole-body PET tracking of a d-dodecapeptide and its radiotheranostic potential for PD-L1 overexpressing tumors.; Authors - Kuan Hu
human	WB,IHC,IF	Mol Cell; Phosphorylated RB Promotes Cancer Immunity by Inhibiting NF-κB Activation and PD-L1 Expression.; Authors - Xin Jin
human	WB,IHC,IF	Mol Ther; Targeting leucine-rich repeat serine/threonine-protein kinase 2 sensitizes pancreatic ductal adenocarcinoma to anti-PD-L1 immunotherapy; Authors - Kang Sun