PD-L1/CD274 Monoklonaler Antikörper

PD-L1/CD274 Monoklonal Antikörper für FC

Wirt / Isotyp

Ratte / IgG2b, kappa

Getestete Reaktivität

Maus und mehr (1)

Anwendung

IHC, FC

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

10F.9G2

Kat-Nr. : 65073-1-Ig

Synonyme

Cd274, PD-L1, Pdcd1lg1, Pdcd1l1, PD L1

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  FC experiment of mouse splenocytes using 65073-1-Ig

  1x10^6 unstimulated or anti-CD3/CD28 stimulated (2 days) mouse splenocytes were surface stained with 0.5 ug Anti-Mouse CD274 (PD-L1, B7-H1) (65073-1-Ig, Clone:10F.9G2) and FITC-anti Rat IgG2b at dilution 1:100 and 0.5 ug CoraLite® Plus 647 Anti-Mouse CD3 (17A2) (CL647-65077, Clone: 17A2). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Anti-CD3/CD28 stimulated mouse splenocytes

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below

Produktinformation

65073-1-Ig bindet in IHC, FC PD-L1/CD274 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Ratte / IgG2b, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: k.A.
• Vollständiger Name: CD274 antigen
• GenBank-Zugangsnummer: BC066841
• Gene symbol: PD-L1
• Gene ID (NCBI): 60533
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Stable for one year after shipment.

Hintergrundinformationen

Programmed cell death ligand 1 (PD-L1, CD274, or B7-H1), is the first member of B7 family to be discovered. B7 family molecules are type I transmembrane proteins belonging to the immunoglobulin superfamily. In concert with their CD28 family receptors, the B7s are key regulators of the adaptive immune response. PD-L1 is suggested as a negative regulator of T and B cell, and plays important role in mediating tolerance of lymphocytes to self-antigens. It is also involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PD-1-independent manner.

Publikationen

Species	Application	Title
human	IHC	J Transl Med; Immunoproteasome subunits are novel signatures for predicting efficacy of immunotherapy in muscle invasive bladder cancer; Authors - XinJian Wang