CD275 (B7-H2) Monoklonaler Antikörper

CD275 (B7-H2) Monoklonal Antikörper für FC

Wirt / Isotyp

Ratte / IgG2a, kappa

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

PE Fluorescent Dye

CloneNo.

HK5.3

Kat-Nr. : PE-65114

Synonyme

AU044799, B7 H2, B7h, B7-H2, B7RP 1, BG071784, CD275, GI50, GL50, GL50 B, ICOS L, icos ligand, Icosl, Icoslg, KIAA0653, LICOS, Ly115l, mKIAA0653

---

Galerie der Validierungsdaten

• 

  FC experiment of mouse splenocytes using PE-65114

  1X10^6 C57BL/6 mouse splenocytes were surface stained with APC Anti-Mouse CD3 (APC-65077, Clone: 17A2) and 0.06 ug PE Anti-Mouse CD275 (B7-H2) (PE-65114, Clone: HK5.3). Cells were not fixed.

• 

  FC experiment of mouse splenocytes using PE-65114

  1X10^6 C57BL/6 mouse splenocytes were surface stained with APC Anti-Mouse CD3 (APC-65077, Clone: 17A2) and 0.06 ug PE-Rat IgG2a isotype control. Cells were not fixed.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Maus-Splenozyten

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

PE-65114 bindet in FC CD275 (B7-H2) und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Ratte / IgG2a, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Maus-B7-RP1-transfizierte Zelllinie
• Vollständiger Name: icos ligand
• GenBank-Zugangsnummer: BC029227
• Gene symbol: Icosl
• Gene ID (NCBI): 50723
• Konjugation: PE Fluorescent Dye
• Excitation/Emission maxima wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: Phosphatbasierter Puffer mit 0,09% Natriumazid und 0,1% Gelatine, pH 7,2.
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD275, also known as B7-H2, ICOSLG or GL50, is a type I transmembrane glycoprotein belonging to the B7 ligand family. CD275 is extensively expressed on professional antigen-presenting cells including B cells, macrophages, and dendritic cells, as well as non-lymphoid cells including mesenchymal stem cells, endothelial cells, fibroblasts, and tumor cells (PMID: 33756276; 35800767). It is a specific ligand for the T-cell-specific cell surface receptor ICOS and acts as a co-stimulatory molecule (PMID: 11023515; 11007762). The interaction of CD275/ICOSLG and ICOS plays important roles in the activation, proliferation, differentiation, and cytokine production of T cells as well as in the antibody secretion from B cells during secondary immune responses (PMID: 21851236).