B7-H3/CD276 Polyklonaler Antikörper

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• KD/KO Validated

B7-H3/CD276 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(19)

Kat-Nr. : 14453-1-AP

Synonyme

CD276, B7-H3, B7H3, B7 homolog 3, B7 H3

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  WB analysis of HEK-293 using 14453-1-AP

  WB result of B7-H3/CD276 antibody (14453-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-B7-H3/CD276 transfected HEK-293 cells.

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  WB analysis using 14453-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14453-1-AP (B7-H3/CD276 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 14453-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14453-1-AP (B7-H3/CD276 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 14453-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14453-1-AP (B7-H3/CD276 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 14453-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14453-1-AP (B7-H3/CD276 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 14453-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14453-1-AP (B7-H3/CD276 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 14453-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14453-1-AP (B7-H3/CD276 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 14453-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14453-1-AP (B7-H3/CD276 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 14453-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14453-1-AP (B7-H3/CD276 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human medulloblastoma using 14453-1-AP

  Immunohistochemical analysis of paraffin-embedded human medulloblastoma using 14453-1-AP (B7-H3/CD276 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human medulloblastoma using 14453-1-AP

  Immunohistochemical analysis of paraffin-embedded human medulloblastoma using 14453-1-AP (B7-H3/CD276 antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, LNCaP-Zellen, U2OS-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe, humanes Medulloblastom-Gewebe, humanes Hodengewebe, humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 10 publications below
• IHC: See 9 publications below
• IF: See 5 publications below
• CoIP: See 1 publications below

Produktinformation

14453-1-AP bindet in WB, IHC, IF, CoIP, ELISA B7-H3/CD276 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: B7-H3/CD276 fusion protein Ag6006
• Vollständiger Name: CD276 molecule
• Berechnetes Molekulargewicht: 57 kDa
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: BC062581
• Gene symbol: CD276
• Gene ID (NCBI): 80381
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

B7-H3 (CD276) is a type I transmembrane protein expressed on many tissues and cell types. B7-H3 is a 100-kDa glycoprotein that belongs to the B7 immunoregulatory family and participates in the regulation of T-cell-mediated immune response probably by functioning as both a T cell costimulator and coinhibitor (PMID: 25567370; 20696859). Overexpressed on a wide range of human solid cancers, B7-H3 has been implicated in cancer progression and metastasis and becomes an attractive target for cancer immunotherapy (PMID: 27208063).

Publikationen

Species	Application	Title
human	WB	J Nutr Biochem; Isothiocyanates attenuate immune checkpoint blockage therapy in gastric cancer via induction of PD-L1 expression; Authors - Qi Zhang
human	WB	J Cell Physiol; Downregulated miR-187 contributes to the keratinocytes hyperproliferation in psoriasis.; Authors - Lipeng Tang
human	IHC	CNS Neurosci Ther; PTX3 mediates the infiltration, migration, and inflammation-resolving-polarization of macrophages in glioblastoma.; Authors - Hao Zhang
human	IHC	J Cancer; The immunoregulatory protein B7-H3 promotes aerobic glycolysis in oral squamous carcinoma via PI3K/Akt/mTOR pathway.; Authors - Zhangao Li
human	IHC	Med Oncol; Overexpression of B7-H3 in CD14+ monocytes is associated with renal cell carcinoma progression.; Authors - Miao Li
human	WB	J Oncol; Alpha-Fetoprotein Regulates the Expression of Immune-Related Proteins through the NF-κB (P65) Pathway in Hepatocellular Carcinoma Cells.; Authors - Qiu-Ting Li