B7-H3 Monoklonaler Antikörper

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• KD/KO Validated

B7-H3 Monoklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human

Anwendung

WB, IP, IHC, IF, FC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

8

CloneNo.

1E7D1

Kat-Nr. : 66481-1-Ig

Synonyme

4Ig B7 H3, B7 homolog 3, B7-H3, CD276, CD276 antigen, CD276 molecule, Costimulatory molecule, UNQ309/PRO352

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 66481-1-Ig

  WB result of B7-H3 antibody (66481-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with normal HEK-293 (sh-control ) and three independent sh-B7-H3 transfected HEK-293 cells.

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  WB analysis of HEK-293 using 66481-1-Ig

  HEK-293, PC-3, LNCaP, HeLa cells, and human spleen, human placenta, pig spleen tissues were subjected to SDS PAGE followed by western blot with 66481-1-Ig (B7-H3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 66481-1-Ig

  Untreated and PNGase F-treated lysates of A431 cells and PC-3 cells were subjected to SDS PAGE followed by western blot with 66481-1-Ig (B7-H3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808).

• 

  IHC staining of human tonsillitis using 66481-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66481-1-Ig (B7-H3 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66481-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66481-1-Ig (B7-H3 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66481-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66481-1-Ig (B7-H3 antibody) at dilution of 1:5000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 66481-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66481-1-Ig (B7-H3 antibody at dilution of 1:750 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 66481-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66481-1-Ig (B7-H3 antibody at dilution of 1:750 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 66481-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66481-1-Ig (B7-H3 antibody at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 66481-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66481-1-Ig (B7-H3 antibody at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66481-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66481-1-Ig (B7-H3 antibody at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66481-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66481-1-Ig (B7-H3 antibody at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human tonsillitis using 66481-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 66481-1-Ig (B7-H3 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of HEK-293 using 66481-1-Ig

  1X10^6 HEK-293 cells were stained with 0.20ug B7-H3 antibody (66481-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, A431-Zellen, PC-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Lungenkarzinomgewebe, humanes Plazenta-Gewebe, humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: humanes Tonsillitisgewebe
• Erfolgreiche Detektion in FC: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:2500-1:10000
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 5 publications below
• IP: See 1 publications below
• FC: See 1 publications below
• CoIP: See 2 publications below

Produktinformation

66481-1-Ig bindet in WB, IP, IHC, IF, FC, CoIP, ELISA B7-H3 und zeigt Reaktivität mit Hausschwein, human

• Getestete Reaktivität: Hausschwein, human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: B7-H3 fusion protein Ag6006
• Vollständiger Name: CD276 molecule
• Berechnetes Molekulargewicht: 57 kDa
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: BC062581
• Gene symbol: CD276
• Gene ID (NCBI): 80381
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

B7-H3 (CD276) is a type I transmembrane protein expressed on many tissues and cell types. B7-H3 is a 100-kDa glycoprotein that belongs to the B7 immunoregulatory family and participates in the regulation of T-cell-mediated immune response probably by functioning as both a T cell costimulator and coinhibitor (PMID: 25567370; 20696859). Overexpressed on a wide range of human solid cancers, B7-H3 has been implicated in cancer progression and metastasis and becomes an attractive target for cancer immunotherapy (PMID: 27208063).

Publikationen

Species	Application	Title
human	WB,FC	J Immunother Cancer; Genome-scale CRISPR-Cas9 screen reveals novel regulators of B7-H3 in tumor cells.; Authors - Shasha Zhao
human	IP	J Transl Med; An N-glycoproteomic site-mapping analysis reveals glycoprotein alterations in esophageal squamous cell carcinoma.; Authors - Yingzhen Gao
human	WB,IHC	J Cancer Res Clin Oncol; B7-H3 and CD47 co-expression in gastric cancer is a predictor of poor prognosis and potential targets for future dual-targeting immunotherapy; Authors - Siji Chen
human	WB,CoIP	J Cancer; B7-H3 inhibits apoptosis of gastric cancer cell by interacting with Fibronectin.; Authors - Meiyun Sun
human	IHC	Int J Gen Med; Comprehensive Analysis Reveals Distinct Immunological and Prognostic Characteristics of CD276/B7-H3 in Pan-Cancer; Authors - Jinye Ding
human	WB,IHC,CoIP	FEBS Open Bio; Fibronectin enhances tumor metastasis through B7-H3 in clear cell renal cell carcinoma; Authors - Jinjing Xie