B7-H3/CD276 Rekombinanter Antikörper

B7-H3/CD276 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

230305A10

Kat-Nr. : 83152-5-RR

Synonyme

4Ig B7 H3, B7 H3, B7 homolog 3, B7H3, CD276, CD276 antigen, CD276 molecule, Costimulatory molecule, UNQ309/PRO352

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  WB analysis using 83152-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83152-5-RR (B7-H3/CD276 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83152-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83152-5-RR against Human CD276 were performed. The affinity constant is 0.607 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: U2OS-Zellen, HEK-293-Zellen, HeLa-Zellen, humanes Plazenta-Gewebe, LNCaP-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83152-5-RR bindet in WB, ELISA B7-H3/CD276 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Fusionsprotein
• Vollständiger Name: CD276 molecule
• Berechnetes Molekulargewicht: 57 kDa
• Beobachtetes Molekulargewicht: 95-100 kDa
• GenBank-Zugangsnummer: NM_001024736
• Gene symbol: CD276
• Gene ID (NCBI): 80381
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

B7-H3 (CD276) is a type I transmembrane protein expressed on many tissues and cell types. B7-H3 is a 100-kDa glycoprotein that belongs to the B7 immunoregulatory family and participates in the regulation of T-cell-mediated immune response probably by functioning as both a T cell costimulator and coinhibitor (PMID: 25567370; 20696859). Overexpressed on a wide range of human solid cancers, B7-H3 has been implicated in cancer progression and metastasis and becomes an attractive target for cancer immunotherapy (PMID: 27208063).