PD-1/CD279 Monoklonaler Antikörper

PD-1/CD279 Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / IgG1, kappa

Getestete Reaktivität

human, nicht-humane Primaten

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

EH12.2H7

Kat-Nr. : 65168-1-Ig

Synonyme

CD279, hPD 1, hPD l, PD1, PDCD1, programmed cell death 1, Protein PD 1, SLEB2

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Galerie der Validierungsdaten

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  FC experiment of human PBMCs using 65168-1-Ig

  1X10^6 day 3 PHA treated human PBMCs were surface stained with 0.2 ug Anti-Human PD-1/CD279 (65168-1-Ig, Clone:EH12.2H7) and CL594-Goat anti-Mouse IgG at dilution 1:1000. Cells were not fixed. Lymphocytes were gated. Blue line indicates unstained stimulated cells. Black dashed line indicates unstimulated stained cells.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: PHA treated human PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

65168-1-Ig bindet in FC PD-1/CD279 und zeigt Reaktivität mit human, nicht-humane Primaten

• Getestete Reaktivität: human, nicht-humane Primaten
• Wirt / Isotyp: Maus / IgG1, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Recombinant human CD279 protein
• Vollständiger Name: programmed cell death 1
• Berechnetes Molekulargewicht: 288 aa, 32 kDa
• GenBank-Zugangsnummer: BC074740
• Gene symbol: PDCD1
• Gene ID (NCBI): 5133
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Mit Protein-A-Affinitätschromatographie gereinigt
• Lagerungspuffer: PBS with 0.09% sodium azide.
• Lagerungsbedingungen: Store at 2-8°C. Stable for one year after shipment.

Hintergrundinformationen

Programmed cell death 1 (PD-1, also known as CD279) is an immunoinhibitory receptor that belongs to the CD28/CTLA-4 subfamily of the Ig superfamily. It is a 288 amino acid (aa) type I transmembrane protein composed of one Ig superfamily domain, a stalk, a transmembrane domain, and an intracellular domain containing an immunoreceptor tyrosine-based inhibitory motif (ITIM) as well as an immunoreceptor tyrosine-based switch motif (ITSM) (PMID: 18173375). PD-1 is expressed during thymic development and is induced in a variety of hematopoietic cells in the periphery by antigen receptor signaling and cytokines (PMID: 20636820). Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function (PMID: 19426218). It is critical for the regulation of T cell function during immunity and tolerance. Blockade of PD-1 can overcome immune resistance and also has been shown to have antitumor activity (PMID: 22658127; 23169436).