CD28 Rekombinanter Antikörper

CD28 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

Biotin

CloneNo.

240354D2

Kat-Nr. : Biotin-98032

Synonyme

Tp44, T-cell-specific surface glycoprotein CD28, CD28 molecule, 240354D2

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  FC experiment of human PBMCs using Biotin-98032

  1x10^6 human PBMCs were surface stained with 0.25 ug Biotin Anti-Human CD28 Rabbit Recombinant Antibody (Biotin-98032, Clone: 240354D2) and CoraLite®488-conjugated streptavidin, or unstained. Cells were then stained with CoraLite® Plus 647 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using Biotin-98032

  1x10^6 human PBMCs were surface stained with 0.25 ug Biotin Anti-Human CD28 Rabbit Recombinant Antibody (Biotin-98032, Clone:240354D2) and CoraLite®488-conjugated streptavidin (red), or unstained (blue). Cells were not fixed. Lymphocytes were gated.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

Biotin-98032 bindet in FC CD28 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Fusionsprotein
• Vollständiger Name: CD28 molecule
• Berechnetes Molekulargewicht: 220 aa, 25 kDa
• GenBank-Zugangsnummer: BC093698
• Gene symbol: CD28
• Gene ID (NCBI): 940
• Konjugation: Biotin
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD28 (T-cell-specific surface glycoprotein CD28), also known as T44 and Tp44, is a 44 kD disulfide-linked homodimeric type I glycoprotein (PMID: 2162180). It is a member of the immunoglobulin superfamily and is expressed on most T lineage cells, NK cell subsets, and plasma cells (PMID: 2162180, 8386518). CD28 may affect in vivo immune responses by functioning both as a cell adhesion molecule linking B and T lymphocytes and as the surface component of a novel signal transduction pathway (PMID: 2162180, 3021470). CD28 binds both CD80 and CD86 with a highly conserved motif MYPPY in the CDR3-like loop (PMID: 15696168, 7964482). CD28 is considered a major co-stimulatory molecule, inducing T lymphocyte activation and IL-2 synthesis, and preventing cell death (PMID: 1348520).