CD28 Rekombinanter Antikörper

CD28 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

241707H6

Kat-Nr. : CL594-98195

Synonyme

241707H6, CD28 antigen, T-cell-specific surface glycoprotein CD28

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  FC experiment of mouse splenocytes using CL594-98195

  1x10^6 mouse splenocytes were surface stained with CoraLite® Plus 488 Anti-Mouse CD3, and 0.25 ug CoraLite®594 Anti-Mouse CD28 Rabbit RecAb (CL594-98195, Clone: 241707H6) or CoraLite®594 Rabbit IgG Isotype Control RecAb (CL594-98136, Clone: 240953C9). Cells were not fixed.

• 

  FC experiment of mouse splenocytes using CL594-98195

  1x10^6 mouse splenocytes were surface stained with 0.25 ug CoraLite®594 Anti-Mouse CD28 Rabbit RecAb (CL594-98195, Clone: 241707H6) or CoraLite®594 Rabbit IgG Isotype Control RecAb (CL594-98136, Clone: 240953C9). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Maus-Splenozyten

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL594-98195 bindet in FC CD28 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD28 fusion protein Eg1408
• Vollständiger Name: CD28 antigen
• Berechnetes Molekulargewicht: 25 kDa
• GenBank-Zugangsnummer: NM_007642.4
• Gene symbol: Cd28
• Gene ID (NCBI): 12487
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD28 (T-cell-specific surface glycoprotein CD28), also known as T44 and Tp44, is a 44 kD disulfide-linked homodimeric type I glycoprotein (PMID: 2162180). It is a member of the immunoglobulin superfamily and is expressed on most T lineage cells, NK cell subsets, and plasma cells (PMID: 2162180, 8386518). CD28 may affect in vivo immune responses by functioning both as a cell adhesion molecule linking B and T lymphocytes and as the surface component of a novel signal transduction pathway (PMID: 2162180, 3021470). CD28 binds both CD80 and CD86 with a highly conserved motif MYPPY in the CDR3-like loop (PMID: 15696168, 7964482). CD28 is considered a major co-stimulatory molecule, inducing T lymphocyte activation and IL-2 synthesis, and preventing cell death (PMID: 1348520).